摘要
目的:制备积雪草苷纳米乳(asiaticoside nanoemulsions,ASI-NEs),优化纳米乳处方,并对其理化性质进行考察。方法:本研究以纳米乳的包封率和溶解度作为评价指标,利用单纯形网格法优化处方,并考察最优处方纳米乳的pH、粒径分布、稳定性等理化性质。结果:ASI-NEs的最优处方组成为:单亚油酸甘油酯、RH 40、二乙二醇单乙醚和水,各部分比例依次为5%,15%,26.3%和53.7%。溶解度和包封率实测值与预测值偏差依次为4.55%和3.69%。最优处方的溶解度为13.97 mg·g^(-1),包封率为90.29%。ASI-NEs为O/W型纳米乳,外观澄清透明,pH值为6.8,平均粒径为(132±5.84)nm,多分散指数(PDI)为0.221 1,具有较好的稳定性,高速离心不分层。结论:本研究成功制备了ASI-NEs,且具有良好的理化性质,为纳米乳载体的进一步应用奠定了基础。
Objective:To prepare the carrier of asiaticoside nanoemulsions(ASI-NEs),optimize the prescription,and evaluate the quality.Methods:The simplex lattice design(SLD)was utilized to screen and optimize the formulation,and the drug solubility and encapsulation efficiency were inspected as evaluation indices.The physicochemical properties such as pH value,particle size distribution and stability of the optimal prescription of ASI-NEs were investigated.Results:Following the optimization,the optimal ASI-NEs formulation was composed of 5%glycerol monooleate,15% Cremophor RH 40,26.3% diethylene glycol monoethyl ether and 53.7%water.The deviations between the measured value and the predicted value of solubility and entrapment efficiency were4.55% and 3.69%,respectively.The solubility and encapsulation efficiency of the optimal prescription were 13.97 mg·g^-1 and 90.29%,respectively.ASI-NEs were O/W nanoemulsion with clear and transparent appearance,pH value of 6.8,average particle size of(132±5.84)nm and PDI of 0.221 1.ASI-NEs had good stability,without delamination under the high-speed centrifugation.Conclusion:In this study,ASI-NEs were successfully prepared.ASI-NEs have good physicochemical properties.This study provided theoretical basis for further study of the carrier of nanoemulsions.
作者
彭倩
李京洋
李慧敏
于子贺
顿珠
张莉
张磊
PENG Qian;LI Jing-yang;LI Hui-min;YU Zi-he;DUN Zhu;ZHANG Li;ZHANG Lei(Pharmaceutical Teaching and Research Department,Logistics University of People's Armed Police Force,Tianjin 300309,China;Department of Pharmaceutics,Affiliated Hospital of Logistics University of People's Armed Police Forces,Tianjin 300162,China)
出处
《中国新药杂志》
CAS
CSCD
北大核心
2019年第10期1276-1280,共5页
Chinese Journal of New Drugs
基金
武警后勤学院基础研究资助项目(WHJ2015012
WHJ201501)
关键词
积雪草苷
纳米乳
单纯形网格法
包封率
理化性质
asiaticoside
nanoemulsions
simplex lattice design (SLD)
entrapment efficiency
physicochemical properties
