摘要
目的研究积雪草苷对高脂饮食诱导高脂血症模型金黄地鼠血清及肝脏内脂质代谢的影响,探讨其血脂调节功能和肝脏保护作用。方法 60只叙利亚金黄地鼠随机分成6组:正常组,模型组,积雪草苷低剂量(15 mg/kg)、中剂量(30 mg/kg)、高剂量(60 mg/kg)组及血脂康(250 mg/kg)组,每组10只,给予相应药物灌胃,正常组和模型组给予等量溶剂,正常组喂养正常饲料,其余各组喂养高脂饲料。均给予4周。测量各组金黄地鼠血清中的总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、谷草转氨酶(AST)、谷丙转氨酶(ALT)水平,以及肝脏组织TC、TG、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)水平,选取各组同部位肝脏组织进行冰冻切片,并通过HE染色观察肝脏结构变化。结果与正常组比较,模型组血清TC、TG、LDL-C、TC/HDL-C、ALT、AST水平以及肝脏TC、TG水平升高(P<0.05,P<0.01),肝脏SOD、GSH-PX水平降低(P<0.01)。与模型组比较,各给药组血清TC、TG、LDL-C、TC/HDL-C水平以及肝脏TC、TG水平降低(P<0.01),肝脏SOD、GSH-PX水平升高(P<0.01),血脂康组与积雪草苷低、中剂量组的血清AST水平降低(P<0.01)。肝组织冰冻切片HE染色显示各给药组肝脏脂肪性病变较模型组减轻。结论积雪草苷可减轻高脂血症模型金黄地鼠的脂肪性病变并缓解肝脏受损程度,其机制可能与增强肝脏抗氧化作用、降低血脂及肝脂有关。
Objective To observe the effect of asiaticoside on blood lipids and liver lipids metabolism in golden hamster model of hyperlipidemia induced by the high-fat diet,and to explore its lipid regulating function and liver protection.Methods Totally 60 Syrian golden hamsters were randomly divided into 6 groups,i.e.,normal group,model group,asiaticoside low dose group(15 mg/kg),asiaticoside middle dose group(30 mg/kg),asiaticoside high dose group(60 mg/kg),Xuezhikang group(250 mg/kg),10 in each group.The normal group and model group were administered with the equal volume of solvent,the other groups were administered with respetive drugs by gastrogavage.The normal group was fed with general diet,and the other group was fed with high-fat diet for 4 weeks.The levels of the total cholesterol(TC),triglyceride(TG),low-density lipoprotein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C),glutamate-private transaminase(ALT),glutamic-oxalacetic transaminase(AST)in serum,as well as the levels of TC,TG,superoxide dismutase(SOD)and glutathione peroxidase(GSH-PX)in liver tissue were measured.The structural changes of frozen section of the same part of liver tissue in all groups were observed by Hematoxylin-Eosin staining.ResultsCompared with the normal group,the serum levels of TC,TG,LDL-C,TC/HDL-C,ALT,AST,and the hepatic levels of TC and TG were increased(P<0.05,P<0.01),the hepatic SOD and GSH-PX were decreased in the model group(P<0.01).Compared with the model group,the serum levels of TC,TG,LDL-C,TC/HDL-C,and the hepatic levels of TC and TG were decreased(P<0.01),the hepatic SOD and GSH-PX increased in the medication groups(P<0.01),the serum levels of AST was decreased in Xuezhikang group,asiaticoside low and middle dose groups(P<0.01).The HE staining results illustrated that fat deposition in the liver was reduced in the medication groups compared with the model group.Conclusion Asiaticoside can reduce fatty lesions and alleviate liver damage in golden hamster hyperlipidemic model,its mechanism may related to stre
作者
林辰曦
陈煜
陈凌
黄萍
余靓平
田维敏
LIN Chen-xi;CHEN Yu;CHEN Ling;HUANG Ping;YU Liang-ping;TIAN Wei-min(Department of Pediatrics,Zhongshan Hospital of Xiamen University,Fujian 361000;Department of Pharmacy,Zhongshan Hospital of Xiamen University,Fujian 361000)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2019年第4期475-479,共5页
Chinese Journal of Integrated Traditional and Western Medicine
关键词
积雪草苷
高脂血症
金黄地鼠
脂质调节
asiaticoside
hyperlipidemia
golden hamster
lipid regulation
