摘要
目的:建立同时测定鼻渊舒口服液中绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素、汉黄芩素含量的方法。方法:采用多波长高效液相色谱法测定绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素和汉黄芩素的含量,色谱柱为Phenomenex Gemini C18色谱柱(4.6 mm×250.0 mm×5μm),甲醇(A)-(体积分数)0.2%磷酸水溶液(B)为流动相,梯度洗脱(0~7 min,10%A;7~12 min,10%~20%A;12~13 min,20%~30%A;13~26 min,30%~65%A;26~35 min,60%~70%A;35~45 min,10%A),流速为1.0 mL·min-1,柱温为40℃,检测波长(0~18.5 min,327nm;18.5~20 min,238n m;20~23 min,321nm;23~45 min,280 nm)。结果:绿原酸、栀子苷、阿魏酸、黄芩苷、黄芩素、汉黄芩素分别在35.2~352.0μg、8.4~84.0μg、1.28~12.8μg、6.0~60.0μg、1.6~16.0μg、1.68~16.8μg范围内线性关系良好;平均回收率依次为绿原酸100.47%(RSD=0.52%),栀子苷101.61%(RSD=1.69%),阿魏酸101.11%(RSD=1.32%),黄芩苷101.26%(RSD=1.24%),黄芩素101.09%(RSD=0.95%),汉黄芩素100.83%(RSD=1.76%)。结论:此方法具有操作快速、简便、重复性好的特点,可用于提高鼻渊舒口服液的质量标准。
Objective:To establish a method for simultaneous determination of chlorogenic acid,geniposide,ferulic acid,baicalin,baicalein and wogonin in Biyuanshu oral liquid.Methods:The contents of chlorogenic acid,geniposide,ferulic acid,baicalin,baicalein and wogonin were determined by multi-wavelength high performance liquid chromatography.The chromatographic column was Phenomenex Gemini C18(4.6 mm×250.0 mm×5 μm).Methanol(A)-(volume fraction)0.2% phosphoric acid aqueous solution(B) was mobile phase,gradient elution(0-7 min,10%A;7-12 min,10%-20%A;12-13 min,20%-30%A;13-26 min,30%-65%A;26-35 min,60%-70%A;35-45 min,10%A),flow rate was 1.0 mL·min-1,column temperature was 40 ℃,detection wavelength(0-18.5 min,327 nm;18.5-20 min,238 nm;20-23 min,321 nm;23-45 min,280 nm).Results:The linear relationships of chlorogenic acid,geniposide,ferulic acid,baicalin,baicalein,and wogonin were good in the range of 35.2-352 μg,8.4-84 μg,1.28-12.8 μg,6-60 μg,1.6-16 μg,1.68-16.8 μg,respectively.The average recovery rate was 100.47% chlorogenic acid(RSD=0.52%);101.61% scorpion glycosides(RSD=1.69%);101.11% ferulic acid(RSD=1.32%);baicalin 101.26%( RSD=1.24%);baicalein 101.09%(RSD=0.95%);wogonin 100.83%(RSD=1.76%).Conclusion:This method has the characteristics of fast operation,simple operation and good repeatability,and can be used to improve the quality standard of Biyuanshu oral liquid.
作者
张一翔
王晓燕
黄霞
李振国
ZHANG Yixiang;WANG Xiaoyan;HUANG Xia;LI Zhenguo(Henan University of Chinese Medicine,Zhengzhou Henan China 450046;Henan Food and Drug Inspection Institute, Zhengzhou Henan China 450000)
出处
《中医学报》
CAS
2019年第5期1034-1038,1047,共6页
Acta Chinese Medicine
基金
河南省科技攻关计划项目(102102310142)
关键词
鼻渊舒口服液
多波长高效液相色谱仪
绿原酸
栀子苷
阿魏酸
黄芩苷
黄芩素
汉黄芩素
Biyuanshu oral liquid
multi-wavelength high performance liquid chromatography
chlorogenic acid
geniposide
ferulic acid
baicalin
baicalein
wogonin
