摘要
以1株产葡萄糖醛酸的鲁氏接合酵母ZSR2为研究对象,通过单因素优化确定了鲁氏接合酵母ZSR2发酵产葡萄糖醛酸的最佳发酵条件(发酵培养基含有80 g/L蔗糖、30 g/L大豆蛋白胨,培养基初始pH 5.0,种龄9 h,接种量为3%)。在此条件下,鲁氏接合酵母ZSR2产葡萄糖醛酸水平提高到14.68 g/L,是优化前的3.8倍。此外,通过在发酵过程中采用补料策略,使葡萄糖醛酸产量进一步提高到 22.36 g/L,是目前纯菌发酵法产葡萄糖醛酸的最高水平。研究结果可为微生物发酵法生产葡萄糖醛酸的工业化进程奠定基础。
The fermentation condition for producing GlcUA by Zygosaccharomyces rouxii ZSR2, a GlcUA producer, was optimized by single factor experiments. It was found that the titer of GlcUA under the optimal fermentation condition (the medium contained 80 g/L sucrose, 30 g/L soy peptone, initial pH=5.0, with 3% inoculum and aged for 9 h) was 14.68 g/L, which was 3.8 times higher than that of unoptimized. Besides, the production of GlcUA enhanced to 22.36 g/L by fed-batch fermentation, which was the highest level reported regarding GlucUA production by single strain fermentation. In conclusion, this study lays a foundation for industrial production of GlcUA by microbial fermentation.
作者
李益烽
方芳
LI Yifeng;FANG Fang(Key Laboratory of Industrial Biotechnology,Ministry of Education (Jiangnan University),Wuxi 214122,China;State Key Laboratory of Food Science and Technology (Jiangnan University),Wuxi 214122,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2019年第10期42-47,共6页
Food and Fermentation Industries
基金
国家轻工技术与工程一流学科自主课题(LITE2018-08)
