摘要
目的:观察黑苦荞茎叶(BBL)对2型糖尿病小鼠治疗作用及其对胰腺、脾脏的影响。方法:将40只SPF级雄性C57/B16小鼠随机分为正常对照组(n=10)和实验组(n=30),实验组给予高糖高脂饲料喂养1个月后,每只小鼠在禁食12h后腹腔注射链脲佐菌素35mg/kg,注射后72h取尾血测血糖≥13.8mmol/L为糖尿病小鼠。成功建立2型糖尿病(T2DM)模型的30只小鼠随机分为3组(n=10):模型对照组(DM组)、低剂量黑苦荞茎叶治疗组(DM+L)组、高剂量黑苦荞茎叶治疗(DM+H)组。对照组和模型组小鼠每日给予生理盐水灌胃,两个黑苦荞茎叶组分别给予0.21g/kg·d-1和0.42g/kg·d-1的黑苦荞茎叶灌胃,14d后杀死小鼠,取血和胰腺、脾脏,检测血清中葡萄糖(FBG)、甘油三酯(TG)、总胆固醇(TCH)、胰岛素和脾脏组织中TNF-α蛋白的含量,观察胰腺组织形态学,称量小鼠体重和脾脏重量、计算脾脏系数,检测胰腺组织中IRS-1mRNA的表达和胰腺组织内胰岛素受体底物IRS-1蛋白表达。结果:与对照组相比,模型组小鼠血清FBG、TC、TCH水平明显升高,使血胰岛素水平明显下降,脾脏组织TNF-α蛋白表达水平显著升高,胰腺组织中IRS-1mRNA表达和IRS-1的蛋白表达水平显著降低(P<0.05);与模型组相比,治疗组小鼠的血清FBG、TC、TCH水平明显降低,血胰岛素水平显著升高,小鼠脾脏系数、脾脏组织TNF-α蛋白表达水平、胰腺组织IRS-1mRNA表达和IRS-1的蛋白表达水平显著升高(P<0.05)。高剂量黑苦荞茎叶(0.42g/kg·d-1)治疗作用更加明显。结论:黑苦荞茎叶对2型糖尿病小鼠具有显著的降血糖、降血脂的治疗效果,对糖尿病小鼠胰腺、脾脏具有一定的保护作用。
Objective:To investigate the therapeutic effects of the black buckwheat leaf(BBL)in type 2 diabetes mellitus mice and its effects on pancreas and spleen.Methods:Forty male C57/B16 mice(SPF)were randomly divided into normal control(NC)group(n=10)and the experimental group(n=30),the experimental group were fed with high sugar and high fat,combined with intraperitoneal injection of streptozotocin in small dose to establish the model of type 2 diabetes mellitus(T2DM).Those thirty model mice were randomly divided into 3 groups(n=10),diabetes mellitus group(DM),low dose of BBL(DM+L)treated group,high dose of BBL(DM+H)treated group.The mice in the NC group and the DM group were given normal saline per day,and the DM+L group and DM+H group were treated with black tartary buckwheat at the doses of 0.21g/kg·d-1 and 0.42g/kg·d-1 respectively.After 14 days.All mice were executed by cervical dislocation,then blood samples were collected,pancreas and spleen were removed for subsequent experiments.The serum levels of fasting blood glucose(FBG),triglyceride(TG),total cholesterol(TCH)and insulin were detected.TNF-αprotein in spleen tissue was detected by ELISA kit.The morphology of pancreas tissue was observed by HE staining,and the spleen coefficient was calculated.The expression levels of insulin receptor substrate-1(IRS-1)mRNA and IRS-1 protein in pancreatic tissue were detected.Results:Compared with the control group,the serum levels of FBG,TC and TCH in the model group were increased significantly,while the serum level of insulin was decreased significantly(P<0.05),the expression of TNF-αprotein in spleen tissues was obviously raised,the expressions of IRS-1 mRNA and IRS-1 protein in pancreatic tissue in model group were decreased significantly(P<0.05).Compared with the model group,the serum levels of FBG,TC and TCH were decreased significantly in the BBL treated groups.The serum insulin level,spleen coefficient,TNF-αprotein expression level in spleen tissue,IRS-1 mRNA expression and IRS-1 protein expression levels in p
作者
项伟玲
金丽琴
高峰
肖敏
陈燕
XIANG Wei-ling;JIN Li-qin;GAO Feng;XIAO Min;CHEN Yan(Department of Biology,School of Laboratory Medicine and Life Science,Wenzhou Medical University,Wenzhou 325035;Department of Acupuncture Massage,Zhejiang Provincial People’s Hospital,People’s Hospital of Hangzhou Medical College,Hangzhou 310014,China)
出处
《中国应用生理学杂志》
CAS
CSCD
北大核心
2019年第2期140-144,共5页
Chinese Journal of Applied Physiology
基金
2015年度浙江省公益技术应用研究计划(实验动物)项目(2015C37099)
关键词
黑苦荞茎叶
2型糖尿病
小鼠
胰腺
脾脏
black buckwheat stem leaf
type 2 diabetes
mice
pancreas
spleen
