摘要
乙肝病毒S蛋白是病毒的包膜蛋白,与病毒进入细胞有关,它存在逆转录过程并且具有极强的潜伏性。本论文应用生物信息学分析乙肝病毒S蛋白的序列特征,利用在线分析软件预测乙肝病毒S蛋白的理化性质和亲疏水性、跨膜区域、信号肽特征、磷酸化位点、二级结构以及乙肝病毒S蛋白的最佳抗原表位形成位置等。结果显示了乙肝病毒S蛋白由226个氨基酸组成,理论等电点是8.21,为不稳定蛋白,总平均亲水性为0.649,是疏水蛋白质,并且该蛋白存在信号肽,有4个跨膜区,有30个潜在的磷酸化位点,主要二级结构为α螺旋和无规则卷曲,同时,结合乙型肝炎病毒S蛋白的序列可及性、线性表位、β转角、柔性、抗原性的预测结果,可以找到潜在的抗原表位区域,为乙型肝炎的表位疫苗研制提供重要的参考依据,有利于进一步对乙型肝炎S蛋白的抗原性进行研究。
S protein in hepatitis B virus is the envelope protein of the virus and is related to the virus entering cell.It has a reverse transcription process and is extremely latent.Bioinformatics analysis tools were used to analyze the sequence characteristics of S protein in hepatitis B virus,and the biological characteristics of S protein virus such as the physicochemical properties,hydrophobicity,transmembrane domains,signal peptides,phosphorylation sites,secondary structures,and optimal epitope formation site were studied.Results showed that the S protein in hepatitis B virus consisted of 226 amino acids,and the theoretical isoelectric point was 8.21.It was an unstable,weak hydrophobic protein with the total average hydrophilicity of 0.649.It had a signal peptide,four transmembrane regions,and 30 potential phosphorylation sites.The main secondary structure of S protein in hepatitis B virus was alpha helix and random coil.The predicted results can be used to find potential epitope regions and provide important reference for the development of epitope vaccines for hepatitis B virus,which is beneficial to the further study of the antigenicity of hepatitis B virus.
作者
李滚
张涛
杨鑫荣
卢香香
LI Gun;ZHANG Tao;YANG Xinrong;LU Xiangxiang(Department of Biomedical Engineering,School of Electronic Information Engineering,Xi an Technological University,Xi an 710021,China)
出处
《生物信息学》
2019年第2期86-94,共9页
Chinese Journal of Bioinformatics
基金
陕西省教育厅专项科学研究计划(No.18JK0377)
