摘要
目的构建汉坦病毒包膜糖蛋白序列数据库,针对汉坦病毒包膜糖蛋白序列进行多态性及正选择位点分析。方法对构建的汉坦病毒包膜糖蛋白序列数据库中所有完整的包膜糖蛋白序列进行比对,采用信息熵评价序列位点的保守性,结合BLOSUM90评分系统和PAML软件包寻找选择压力下的异常氨基酸替换模式。结果汉坦病毒包膜糖蛋白中G1第11位、72位、204位和G2第8位和346位点等10个位点氨基酸替换差异有统计学意义。汉坦病毒各亚型之间未发现有意义的正选择位点。SEO型汉坦病毒G2糖蛋白第438位氨基酸位点为正选择位点。结论通过汉坦病毒包膜糖蛋白序列数据库,用生物信息学方法不仅能够检验已知生物学特性蛋白各功能域的保守性,更可为潜在的新功能域的研究提供切入点。
Objective To study the polymorphism of hantavirus envelope glycoprotein from available database.Methods The sequences of the hantavirus envelope glycoprotein were obtained from the NCBI database and the software Shannon entropy was used for evaluation.Amino acid substitution patterns that reflect specific selective pressure were searched and identified by using BLOSUM90 scoring matrix and PAML software package.Results Among all amino acid substitution patterns,amino acid sites at 11,72,204,225,247,324,427 and 644 of the glycoprotein G1 and at 8 and 346 of the glycoprotein G2 showed highly statistical significance.No significant positive selection sites were found among various subtypes of hantavirus but only one positive selection site at amino acid site 438 of the SEO glycoprotein G2.Conclusions The amino acid conservation and the described functional domains are closely associated of different subtypes of hantavirus.Several structural domains were predicted to have potential functions which could be further studied.
出处
《中国病毒病杂志》
CAS
2012年第5期383-387,共5页
Chinese Journal of Viral Diseases
