摘要
以天然花生为原料,冷榨去油并粉碎之后,通过浸提获得Ara h6粗蛋白液,再采用阴离子交换柱层析和分子筛凝胶过滤法进一步纯化,使用SDS-聚丙烯酰胺凝胶电泳和飞行时间串联质谱对Ara h6蛋白进行鉴定。结果表明:分离纯化出的Ara h6蛋白纯度达到95%以上。
Natural peanuts were used as raw materials,removed oil by cold pressed and crushed into powder.Ara h6 crude protein solution was obtained by extraction and isolated,and puri fied by anion exchange column chromatography and molecular sieve gel filtration method.The Ara h6 protein was identified by SDS-polyacrylamide gel electrophoresis and time-of-flight tandem mass spectrometry.The results showed that the purity of the puri fied Ara h6 protein was above 95%.
作者
夏潇潇
付岩
王飞
孙季
张鹏
XIA Xiao-xiao;FU Yan;WANG Fei;SUN Ji;ZHANG Peng(Department of Biology & Environment Engineering,Hefei University,Hefei 230601,Anhui,China2. Anhui Huizhirun Food Co.,Ltd.,Huainan 237000,Anhui,China)
出处
《粮食与油脂》
北大核心
2019年第6期12-14,共3页
Cereals & Oils
基金
安徽高校自然科学研究重点项目(KJ2017A546)
安徽省2019年重点研究与开发计划项目(201904a06020003)
关键词
花生
ARA
h6蛋白
粗提
纯化
peanut
Ara h6 protein
crude extraction
puri fication
