摘要
目的:研究甲状旁腺激素(PTH)对下颌升支截骨术后正畸牙移动过程中骨保护素(OPG)和核因子κB受体活化因子配体(RANKL)的表达。方法:48只兔建立下颌升支截骨和下颌第一磨牙正畸移动模型,随机分成2组。实验组术后间歇性注射PTH 20μg/kg,对照组注射生理盐水。免疫组化方法和荧光定量PCR检测正畸牙牙周组织中RANKL和OPG表达变化。结果:实验组正畸牙压力侧牙周组织RANKL蛋白及mRNA表达大于对照组(P<0.05),而OPG蛋白及mRNA表达均小于对照组(P<0.05)。结论:外源性PTH可通过促进正畸牙压力侧牙周组织中RANKL表达,抑制OPG的表达,加速下颌升支截骨术后正畸牙的移动。
Objective: To investigate the expression of osteoprotegerin(OPG) and receptor activator of nuclear factor-κB ligand(RANKL) in orthodontic tooth movement after mandibular ramus osteotomy regulated by parathyroid hormone(PTH). Methods: An orthodontic tooth movement model of mandibular first molars after mandibular ramus osteotomy was established in 48 rabbits, then the rabbits were randomly divided into 2 groups. The rabbits in experimental group were administered intermittent subcutaneous injection of 20 μg/kg PTH, those in the control group were given normal saline. RANKL and OPG expression in the periodontal tissues of the molars was examined by immunohistochemistry and q-PCR. Results: Experimental group exhibited significantly higher RANKL mRNA level and lower OPG mRNA level on the compression side of periodontal tissues than the control group. Conclusion: Administration of PTH can accelerate the movement of orthodontic tooth after mandibular ramus osteotomy by promoting the expression of RANKL and inhibiting the expression of OPG in periodontal tissue.
作者
李耀
唐正龙
陈小燕
王冬香
高琼
LI Yao;TANG Zhenglong;CHEN Xiaoyan;WANG Dongxiang;GAO Qiong(Department of Oral and Maxillofacial Surgery,Stomatological Hospital of Guizhou Medical University,550004 Guiyang,China)
出处
《实用口腔医学杂志》
CAS
CSCD
北大核心
2019年第3期371-375,共5页
Journal of Practical Stomatology
基金
国家自然科学基金(编号:81460101)
