摘要
目的比较5种方法及8种试剂对艾滋病(HIV)早期感染者的血清动态检测,探讨HIV早期感染血清初筛检测的最佳方案。方法应用3代ELISA、4代ELISA、胶体硒标、化学发光4代试剂及免疫印迹法(Westernblot)5种方法,对3例HIV早期感染者血清进行追踪检测,分析其不同时期血清检测的动态变化。结果5种方法的阳性检出率排序依次为化学发光4代试剂最高,其次为4代ELISA和胶体硒,3代ELISA、Westernblot最低。结论初筛实验室应该用2种方法同时进行HIV抗体检测,以此排除不同试剂灵敏性差异的缺陷,或可应用4代抗原抗体联合检测试剂进行初筛,对不确定或可疑样本不轻易报告阴性,进一步用蛋白印迹法或核酸检测进行辅助诊断。
Objective To compare the five methods and eight reagents in detection of early HIV infection, and discuss a better method for HIV screening. Methods Using third generation ELISA, fourth generation ELISA and quick test reagent, chemiluminescence immunoassay and Western blot were used to track detection of serum in three cases of early HIV infection and analyze the dynamic change of serum in different periods. Results The positive detection rate of the five methods was the highest in the fourth generation of chemiluminescence, followed by the fourth generation ELISA and colloidal selenium, and the third generation ELISA and Western blot were the lowest. Conclusion The screening laboratory should use two methods to detect HIV antibody simultaneously to eliminate the defects caused by sensitivity differences between different reagents, or use the 4th generation antigen - antibody combined detection reagent for primary screening. The uncertain or suspicious samples should not be reported as Negative until further diagnosed by Western blot or nucleic acid detection.
作者
林琳
朱鸿
程艳杰
赵丹
LIN Lin;ZHU Hong;CHENG Yan -jie;ZHAO Dan(Clinical Laboratory, the First Affiliated Hospital of Dalian Medical University, Dalian, Liaoning 116011, China)
出处
《中国卫生检验杂志》
CAS
2019年第10期1270-1272,共3页
Chinese Journal of Health Laboratory Technology
