摘要
目的探讨巴豆醛亚慢性染毒对雄性大鼠的生殖损伤和氧化损伤机制的影响。方法将40只SPF级雄性Wistar大鼠按随机数字表法分为对照组和3个染毒组,每组10只。对4组大鼠进行巴豆醛(生理盐水配制)连续灌胃染毒,1次/d,持续128 d,染毒剂量分别为0.0、2.5、4.5、8.5 mg/kg。染毒结束后称量大鼠体重、睾丸及附睾重量,并计算脏器系数;取左侧附睾尾测定大鼠精子活率、数量以及睾丸组织标志酶活力[乳酸脱氢酶(LDH)、山梨醇脱氢酶(SDH)、酸性磷酸酶(ACP)、γ-谷氨酰转肽酶(γ-GT)];测定血清中卵泡刺激素(FSH)、促黄体生成素(LH)、睾酮(T)浓度以及氧化应激相关指标浓度[丙二醛(MDA)、超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)和过氧化氢酶(CAT)]。结果与对照组比较,4.5 mg/kg和8.5 mg/kg染毒组大鼠体重增加量、睾丸和附睾重量以及脏器系数均下降,差异均有统计学意义(P<0.05)。染毒组大鼠睾丸组织体积缩小、颜色暗深,部分生精小管内生殖细胞数量减少。与对照组比较,4.5 mg/kg和8.5 mg/kg染毒组精子数量和精子活力明显降低,差异有统计学意义(P<0.05);与对照组比较,4.5 mg/kg和8.5mg/kg染毒组大鼠血清中ACP、LDH、SDH和γ-GT活力降低,差异均有统计学意义(P<0.05);与对照组比较,8.5 mg/kg染毒组大鼠血清中T水平降低,4.5 mg/kg和8.5 mg/kg染毒组LH和FSH水平降低,差异均有统计学意义(P<0.05);与对照组比较,4.5 mg/kg和8.5 mg/kg染毒组大鼠血清中MDA活力升高,SOD、GSH-Px和CAT活力降低,差异均有统计学意义(P<0.05)。结论巴豆醛可能改变大鼠生殖系统激素含量、抗氧化酶表达,破坏机体氧化平衡状态,造成大鼠亚慢性生殖损伤和氧化损伤。
Objective To investigate the effect of subchronic exposure to crotonaldehyde on reproductive damage and oxidative stress in male rate. Methods Forty male SPF Wistar rats were randomly divided into four group: a control group and 3 exposure groups, 10 per group. The rats in each group were continuously administrated with crotonaldehyde (normal saline) for 1 time/d. For 128 d, the doses were 0.0, 2.5, 4.5, 8.5 mg/kg. After the end of the exposure, the body weight, the weight of the testis and epididymis was measured, and calculating organ coefficient. The left spermatozoon tail was used to determine sperm motility, number and testicular tissue marker enzyme activity : LDH, SDH, ACP,γ-GT;blood biochemical related index concentration: FSH, LH, T;oxidative stress-related indicator concentrations: MDA, SOD, GSH-Px and CAT. Results Compared with the control group, the weight gain, testicular and epididymis weight, and organ coefficient of the rats in the 4.5 and 8.5 mg/kg groups were decreased, the difference was statistically significant (P<0.05). In the exposed group, the testicular tissue volume was reduced, the color was dark, and the number of germ cells in some seminiferous tubules was reduced. Compared with the control group, the sperm count and sperm motility of the 4.5 and 8.5 mg/kg groups were significantly lower (P<0.05);compared with the control group, 4.5 and 8.5 mg/kg. The activities of serum ACP, LDH, SDH and γ-GT in the exposed group were significantly lower (P<0.05). Compared with the control group, the serum levels of T in the 8.5 mg/kg group were decreased. The levels of LH and FSH in the 4.5 and 8.5 mg/kg exposure groups were significantly lower (P<0.05). Compared with the control group, the rats in the 4.5 mg/kg and 8.5 mg/kg exposure groups were compared. The activity of MDA in serum increased, SOD, GSH-Px and CAT activity decreased, the difference was statistically significant (P<0.05). Conclusion Crotonaldehyde may cause subchronic reproductive damage and oxidative damage in rats by altering th
作者
李双双
张彪
张志虎
Li Shuangshuang;Zhang Biao;Zhang Zhihu(School of Medicine and life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan 250062, China;Shandong Academy of Occupational Health and Occupational Medicine, Jinan 250062, China)
出处
《中华劳动卫生职业病杂志》
CAS
CSCD
北大核心
2019年第4期241-246,共6页
Chinese Journal of Industrial Hygiene and Occupational Diseases
基金
山东省自然科学基金(ZR2016YL016).
关键词
巴豆醛
大鼠
生殖损伤
氧化性应激
Crotonaldehyde
Rats
Reproductive damage
Oxidative stress
