摘要
以硬毛粗盖孔菌(Funalia trogii)为研究对象,考察培养基组成及培养条件等对其发酵产漆酶的影响,并进一步研究漆酶对食用合成色素日落黄的降解脱色效果。结果表明,最适培养基组成及培养条件为:玉米淀粉25 g/L,蛋白胨2 g/L,Cu^(2+)1.5 mmol/L,香兰素1 mmol/L,培养温度30℃、初始p H6.0,摇床转速200 r/min,硬毛粗盖孔菌培养8 d后发酵液漆酶活力为(329±12) U/m L,是初始培养条件下的11.96倍。漆酶粗酶液对日落黄呈现出较高的降解脱色作用并可耐受高浓度底物(800 mg/L),50～200 mg/L日落黄脱色率均可达90%以上,漆酶介体ABTS可提升对日落黄脱色效率及速率,并降低高浓度底物对漆酶的抑制作用,曲酸的加入则抑制了漆酶活性,导致漆酶对日落黄的脱色率仅为7.14%。本研究为漆酶资源在食品行业的深入开发与应用提供理论依据。
The effect of composition of medium and culture conditions on the laccase production by Funalia trogii was evaluated,and the degradation of edible synthetic pigment sunset yellow by crude laccase was further studied. The results showed that the optimum culture conditions were as follows: 25 g/L corn flour,2 g/L peptone,Cu^2+ 1.5 mmol/L,1 mmol/L vanillin,culture temperature 30 ℃,initial pH6.0,rotation speed 200 r/min.Under the conditions of fermentation,the activity of laccase from F.trogii after cultured for eight days was( 329 ± 12) U/m L,which was 11.96-fold compared to the control.Crude laccase showed high efficiency degradation of sunset yellow and could tolerate high concentration of substrate( 800 mg/L).Decolorization efficiency for 50~200 mg/L sunset yellow more than 90%.The addition of laccase mediator could significantly increase the efficiency and rate of sunset yellow decolorization,meanwhile,decrease the inhibition of laccase by high concentration substrates.The addition of kojic acid inhibited the activity of laccase,then the decolorization rate of sunset yellow by laccase was only 7.14%.This study provides a theoretical basis for further development and application of laccase resources in the food industry.
作者
宋丽丽
张志平
王光路
杨旭
张靖楠
SONG Li-li;ZHANG Zhi-ping;WANG Guang-lu;YANG Xu;ZHANG Jing-nan(College of Food and Biological Engineering,Zhengzhou University of light Industry,Zhengzhou 450002,China)
出处
《食品工业科技》
CAS
北大核心
2019年第10期133-139,共7页
Science and Technology of Food Industry
基金
国家自然科学基金(21706244)
河南省科技攻关(152102110104)
郑州轻工业学院博士基金(2013BSJJ005)
关键词
漆酶
硬毛粗盖孔菌
日落黄
脱色
laccase
Funalia trogii
sunset yellow
decolorization
