摘要
随着我国印染工业的发展,废水对生态环境的危害日趋严重,亟需开发一种脱色明显且成本低廉的降解方法。本研究发现毛木耳Auricularia cornea菌株AC5对不同结构的染料均具有一定的降解作用,尤其是三苯甲烷类染料。利用26℃、160r/min振荡培养7d的粗酶液对染料(75.0mg/L)进行12h降解,结果显示三苯甲烷染料孔雀石绿、结晶紫,蒽醌染料活性蓝19和偶氮染料活性蓝222的降解效率分别为83.27%、71.77%、67.81%和63.92%。染料降解实验和酶活力测定结果表明,毛木耳对孔雀石绿的降解率达到最高时漆酶活性最高,为321.0U/mL,木质素过氧化物酶和锰过氧化物酶活性较低。因此,推测在降解过程中漆酶起到主要作用。研究表明利用毛木耳菌丝发酵液降解染料废水成本低且操作方便,为染料废水的降解研究提供了前期基础。
With the development of printing and dyeing industry in China,the harmfulness of wastewater to the ecological environment is becoming increasingly serious,and it is urgent to explore a degradation method with obvious decolorization and low cost.It was found that Auricularia cornea strain AC5 could degrade various types of dyes,especially triphenylmethane dye.Degradation experiment of different dyes(75.0mg/L)was carried out for 12h using the extracellular crude enzyme solution of A.cornea incubated in 7d at 26°C under the condition of shake culture(160r/min).The result showed that the degradation rates of triphenylmethane dye malachite green,crystal violet,anthraquinone dye reactive blue 19 and azo dye active blue 222 were 83.27%,71.77%,67.81%and 63.92%,respectively.Enzyme activity determination showed that the laccase activity reached up to 321.0U/mL when degradation rate of malachite green attained to the highest level.The activities of lignin peroxidase and manganese peroxidase were lower than that of laccase.It was inferred that laccase played a major role in the process of dyes’degradation.This study proved that it was a low-cost and convenient method using the crude enzyme solution of A.cornea to degrade dye wastewater.
作者
柏晓冉
朱孟娟
张少岩
周佳
李晓博
李壮
孟丽
BAI Xiao-Ran;ZHU Meng-Juan;ZHANG Shao-Yan;ZHOU Jia;LI Xiao-Bo;LI Zhuang;MENG Li(Shandong Provincial Key Laboratory of Agricultural Microbiology,College of Plant Protection,Shandong Agricultural University,Tai’an,Shandong 271018,China;Shandong Provincial Key Laboratory for Biology of Vegetable Diseases and Insect Pests,College of Plant Protection,Shandong Agricultural University,Tai’an,Shandong 271018,China;Shandong Mushroom Industrial Technology Innovation Research Institute,Zoucheng,Shandong 273500,China)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第6期1175-1186,共12页
Mycosystema
基金
国家重点研发计划项目(2018YFD1001001)
山东省泰山产业领军人工程项目(LJNY201611)
邹城市科技创新创业领军人才专项经费资助项目。
