摘要
目的:探讨跨膜蛋白106A(transmembrane protein 106A,TMEM106A)在肺癌肿瘤中的表达及其与临床病理特征的关系,并进一步研究了该分子对肺癌细胞增殖的影响。方法:收集肺癌病人手术切除标本31例,提取组织RNA,采用荧光实时定量PCR实验,检测肺癌组织以及肺癌旁组织中TMEM106A mRNA的相对表达量。构建TMEM106A慢病毒表达载体Plenti-UBI/TMEM106A,转染至H1299细胞中,实现TMEM106A高表达,采用CCK-8细胞增殖实验和克隆形成实验观察TMEM106A过表达对肺癌细胞H1299 增殖能力的抑制作用。结果:荧光实时定量PCR实验结果显示肺癌组织中,TMEM106A基因的mRNA相对表达量低于肺癌旁对照组。成功构建肺癌H1299-TMEM106A过表达细胞系,与H1299-control比较,细胞系H1299-TMEM106A中TMEM106A表达显著升高;增殖实验结果显示TMEM106A高表达显著抑制了肺癌细胞的增殖能力;克隆形成实验结果显示细胞系H1299-TMEM106A中高表达TMEM106A的肺癌细胞克隆形成能力明显低于H1299-control肺癌细胞( P < 0.01 )。结论:TMEM106A能抑制肺癌细胞的增殖。
AIM : To investigate the expression of transmembrane protein 106A (TMEM106A) in lung cancer and its relationship with clinicopathological features,and the effect of this molecule on the proliferation of lung cancer cells. METHODS : Thirty-one cases of lung cancer tissues were collected from patients with early stage lung cancer.Tissue RNA was extracted and real-time quantitative PCR was used to detect the relative expression of TMEM106A mRNA in lung cancer tissues and para-cancer tissues.TMEM106A lentiviral expression vector Plenti-UBI/TMEM106A was constructed and transfected into H1299 cells to overexpress TMEM106A.Cell proliferation assay and colony formation assay were used to observe the effect of TMEM106A overexpression on the proliferation of lung cancer H1299 cells. RESULTS :Real-time quantitative PCR (qRT- PCR) showed that the relative expression of TMEM106A gene mRNA in lung cancer tissues was lower than that in the control para-cancer tissues.H1299-TMEM106A,the cell line overexpressed TMEM106A,was successfully constructed.Compared with H1299-control,the expression of TMEM106A in cell line H1299-TMEM106A was significantly increased.The proliferation experiment showed that the high expression of TMEM106A significantly inhibited the proliferation of lung cancer cells.The colony formation assay showed that the colony forming ability of lung cancer cells with high expression of TMEM106A in cell line H1299-TMEM106A was significantly lower than that of H1299-control lung cancer cells ( P < 0.01 ).CONCLUSION : TMEM106A can inhibit the proliferation of lung cancer cells.
作者
潘欢
周玉玲
戚维波
沈辉
金晶
沈建芬
马兴杰
徐玉芬
PAN Huan;ZHOU Yuling;QI Weibo;SHEN Hui;JIN Jing;SHEN Jianfen;MA Xingjie;XU Yufen(The First Hospital of Jiaxing,Jiaxing 314000,Zhejiang,China;Featured Medical Center of Chinese People's Armed Police Force,Beijing 102613,China)
出处
《中国临床药理学与治疗学》
CAS
CSCD
2019年第5期529-534,共6页
Chinese Journal of Clinical Pharmacology and Therapeutics
基金
浙江省中医药科技计划项目(2015ZQ034)
浙江省医药卫生科技计划(2018KY800)
嘉兴市科技计划项目(2017AY33004)
嘉兴市第一医院院级课题(2018-YA-35)
嘉兴市第一医院院级课题(2018QMY006)
