摘要
为快速确诊肉牛牛支原体病,根据牛支原体 OPPD/F 基因序列设计1对引物,通过反应条件的优化,建立牛支原体PCR诊断方法。结果表明:该方法可从牛肺、鼻拭子临床样本和其临床样本的培养物中直接检测到牛支原体病病原核酸,该方法可检测的病原核酸最小浓度约为1×10^-7 μg,整个检测过程仅需3.5 h。该方法具有快速、灵敏性高、特异性强等特点,对牛支原体感染的诊断、防治与预后评估具有重要意义。
To rapidly diagnose Mycoplasma bovina in beef cattle,a pair of primers were designed according to the OPPD/F gene sequence of M.bovina ,and the PCR diagnosis method of M.bovina was established by optimizing the reaction conditions.Results:The method could detect the pathogenic nucleic acid of M.bovina directly from the clinical samples of bovine lung and nose swabs and the culture of the clinical samples.Minimum detectable concentration was 1×10^-7 μg,and the detecting time was 3.5 h.It was concluded that the established PCR assay was rapid,highly specific and sensitive,which could be important in disease diagnosis,control applications and prognostic estimation of M.bovina.
作者
吴位珩
徐景峨
余波
张涛
余国富
杨莉
冯明祥
孙启跃
刘镜
黄波
杨茂生
姜玲玲
WU Weiheng;XU Jing′e;YU Bo;ZHANG Tao;YU Guofu;YANG Li;FENG Mingxiang;SUN Qiyue;LIU Jing;HUANG Bo;YANG Maosheng;JIANG Lingling(Institute of Animal Husbandry and Veterinary Medicine,Guizhou Academy of Agricultural Sciences,Guiyang,Guizhou 550005;Animal Disease Control and Prevention Centre of Qingzhen City,Qingzhen,Guizhou 551400;Animal Husbandry Service Center of Guanling Autonomous County in Guizhou Province,Guanling,Guizhou,561300,China)
出处
《贵州农业科学》
CAS
2019年第5期66-69,共4页
Guizhou Agricultural Sciences
基金
贵州省农业攻关计划项目“肉牛支原体病防控关键技术研究”[黔科合支撑(2016)2503]
贵州省农业产业技术体系建设专项“贵州省现代肉牛产业技术体系建设项目”(GZCYTX-0301)
贵州省工程技术研究中心建设项目“贵州省肉牛养殖工程技术研究中心建设项目”[黔科合平台人才(2016)5201]
贵州省农业领域科技重点项目“贵州优质专用肉牛品种选育”[黔科合NY(2015)3002]
贵州省科技厅创新能力建设项目“贵州省肉牛现代农业产业技术体系建设项目”(GZCYTX2014-03)
