摘要
实验以虹鳟为研究对象,选取17种中草药开展中草药免疫增强剂的体外快速筛选研究。采用Percoll密度梯度离心方法,对虹鳟外周血白细胞进行分离纯化实验。结果表明:白头翁、桂桔、白芨、大黄、贯众、红景天6种中草药的SPO显著高于对照组(P<0.05),且NBTN显著提高[NBTB(%)>15%];白芨、厚朴、麻黄、丹参、大黄、马齿苋、泽泻、黄柏、苍耳子、贯众、红景天组的吞噬活性(PA)值显著高于对照组(P<0.05),蒲公英组的吞噬指数(PI)值显著高于对照组(P<0.05)。综合上述评价指标:白芨、大黄、贯众、红景天可作为虹鳟免疫增强剂的备选中草药。
This research was conducted to deter? mine 17 Chinese herbal medicines were selected for rapid screening of immunostimulants of O. mykiss in vitro. Percoll density gradient centrifugation methods was used to isolate and purify white blood cells from peripheral blood of O. mykiss . The results showed that SPO-values of Anemone chinensis, Cassia twig, Rhizoma bletil? lae, Radixet rhizomarhei, Cyrtomium rhizome and Rhodiola rosea were significantly highest compared with control(P<0.05), and NBTB values were significantly improved[NBTB(%)>15%]. That PA values of Rhizoma bletillae, Cyrtomium rhizome, Rhodiola rosea, Cortex phellodendri, Fructus xanthii, Radix et rhizoma rhei, Port ulaca oleracea L, Rhizoma alismatis, Herba ephedrae, Radix salviae miltior? rhizae and Magnolia bark were significantly highest compared with control group(P<0.05), the PI values of Taraxacum mongolicum were significantly highest compared with control group(P<0.05). Overall, four kinds of Chinese herbal medicines such as white Radix Paeoniae Alba, Radix et rhizoma rhei, Cyrtomium rhizome, and Rhodiola rosea can be used as immunostimulants forO. mykiss.
作者
董春燕
姚丽
邵俭
安苗
文明
姜海波
Dong Chunyan;Yao Li;Shao Jian;An Miao;Wen Ming;Jiang Haibo
出处
《饲料工业》
北大核心
2019年第10期53-56,共4页
Feed Industry
基金
贵州省科技支撑计划项目[黔科合支撑[2016]2502号
[2016]2511号
[2017]2532号]
贵州省科技平台及人才团队计划[黔科合平台人才(2018)5253]
国家现代农业产业技术体系专项资金[CARS-46]
北京市鲟鱼
鲑鳟鱼创新团队项目[BAIC08-2018]
关键词
中草药
虹鳟
免疫增强剂
氧呼吸爆发活性
吞噬活性
Chinese herbal medicine
Oncorhynchusmykiss
immunostimulants
oxygen respiratory burst activity
phagocytic activity
