摘要
[目的]分析我国7个地理种群小鼠TLR9基因的遗传多样性。[方法]利用PCR技术扩增并测序7个地理种群100个小鼠样品的TLR9基因。使用MEGA7.0、DnaSP等软件对基因序列进行分析,计算遗传距离、遗传多样性,并构建系统发育树。[结果]获得的TLR9基因片段长度为1030bp,位于参与对病原体相关分子模式识别的LRR结构域的编码区,A、T、C、G的平均含量分别为22.9%、22.2%、32.8%和22.0%,各地理种群间碱基含量没有明显差异。通过计算各地理种群间遗传距离及核苷酸多样性发现,这7个地理种群小鼠TLR9基因序列较为保守,并无明显遗传分化。以褐家鼠(NM_198131)作为外群构建系统发育树,其结果与遗传多样性分析相一致。[结论]7个地理种群小鼠TLR9基因遗传多样性较低,具有极高的保守性。
[Objective]To analyze the genetic diversity of TLR9 gene in Mus musculus from 7 geographical populations of China.[Method] PCR technology was used to amplify and sequence TLR9 gene of 100 mice samples in 7 geographical populations.Using MEGA 7.0,DnaSP and other software,the sequences of TLR9 gene were analyzed,the genetic distance and genetic diversity were calculated,and the phylogenetic tree was constructed.[Result]The length of TLR9 gene fragment was 1 030 bp. TLR9 gene was located in the encoding region of LRR domain, which was involved in the identification of pathogen-related molecular patterns.The average content of bases A,T,C,G were 22.9%,22.2%,32.8% and 22.0% respectively.There was no significant difference in base composition among different geographical populations.Through calculating the genetic distance and nucleotide diversity among the geographical populations,it was found that TLR9 gene in M.musculus in 7 geographical populations was conservative,and there was no obvious genetic differentiation.The phylogenetic tree was constructed with Rattus norvegicus (NM_198131) as the outgroup,the results were consistent with the genetic diversity analysis.[Conclusion]The genetic diversity of TLR9 gene in M.musculus of 7 geographical populations was low,with high conservation.
作者
冷冰霜
于立强
黄玲
LENG Bing-shuang;YU Li-qiang;HUANG Ling(College of Life Sciences,Ludong University,Yantai,Shandong 264025;Yantai No.3 Middle School,Yantai,Shandong 264000)
出处
《安徽农业科学》
CAS
2019年第10期89-91,共3页
Journal of Anhui Agricultural Sciences
基金
国家自然科学基金项目(31371252)
