摘要
背景:低水平表达的脂联素在牙周炎和糖尿病的发生与发展进程之中均发挥着明显的促进作用。目的:探讨脂联素注射对糖尿病并发牙周炎病理模型小鼠的治疗作用及其机制。方法:60只8周龄的昆明系小鼠,由北京维通利华动物实验技术有限公司提供。小鼠被平均随机分为3组,其中模型组和脂联素组中所有小鼠均接受一次性的链脲佐菌素腹腔注射从而建立小鼠糖尿病模型,于牙周结扎部位涂抹联合菌液以建立小鼠牙周炎模型。正常对照组和糖尿病牙周炎模型组小鼠每日1次接受1mL生理盐水腹腔注射,脂联素组小鼠则每日接受1次15μg/kg的脂联素腹腔注射。治疗持续6周,酶联免疫吸附法(ELISA)检测小鼠血清及牙周组织中脂联素的水平、血糖及血浆胰岛素的水平;观察小鼠的牙周探诊深度以及牙槽骨垂直吸收高度;苏木精-伊红染色检测牙周组织病理变化;TUNEL法检测牙龈上皮细胞凋亡水平变化,RT-PCR和Western blot检测RAGE、活性氧基因和蛋白表达。结果与结论:①脂联素组小鼠的血清和牙周组织中的脂联素水平较正常对照组和模型组明显升高(P<0.05);②脂联素组较模型组小鼠血糖水平明显降低(P<0.05),血清胰岛素水平明显升高(P<0.05);③脂联素组小鼠的牙周探诊深度和牙槽骨垂直吸收高度较模型组明显减轻,牙龈上皮细胞凋亡率也明显降低;④RAGE、活性氧基因和蛋白表达水平脂联素组较模型组明显减轻(P<0.05);⑤结果说明,脂联素干预可以提高糖尿病牙周炎病理模型小鼠牙周组织及血浆的脂联素水平,并可以明显改善小鼠的血糖状态;减轻小鼠牙周组织的病理变化及牙周细胞的凋亡,此系通过调节RAGE-ROS信号通路发挥以上调节作用。
BACKGROUND:Low level of adiponectin plays a significant role in the occurrence and development of periodontitis and diabetes mellitus.OBJECTIVE:To investigate the therapeutic effect of adiponectin injection on diabetic periodontitis model mice and its mechanism.METHODS:Sixty 8-week old Kunming mice(provided by Beijing Weitong Lihua Animal Experimental Technology Co.,Ltd.)were randomly divided into three groups:control group,model group and adiponectin group.The mice in the model and adiponectin groups received intraperitoneal injection of streptozotin to establish a mouse model of diabetes mellitus,and then the periodontitis model was established by applying the combined bacterial fluid to the ligation site.The mice in the control and model groups received intraperitoneal injection of 1 mL normal saline once daily,while adiponectin group received intraperitoneal injection of 15 μg/kg adiponectin once daily,for 6 consecutive weeks.The three experimental groups of mice were treated for 6 weeks,after which physiological and biochemical indicators were tested.The levels of adiponectin in serum and periodontal tissue,the blood sugar and plasma insulin levels were then detected by ELISA.The depth of periodontal examination and the vertical absorption of alveolar bone were observed.The pathological changes of periodontal tissue were detected by hematoxylin-eosin staining.The changes of apoptosis level of gingival epithelial cells were detected by TUNEL method.The expression levels of RAGE and reactive oxygen species mRNA and protein were detected by RT-PCR and western blot assay.RESULTS AND CONCLUSION:(1)The levels of adiponectin in serum and periodontal tissue in the adiponectin group were significantly higher than those in the control and model groups(P<0.05).(2)The blood sugar level in the adiponectin group was significantly lower than that in the model group(P<0.05)and the serum insulin level was significantly increased(P<0.05).(3)The depth of periodontal examination and vertical absorption of alveolar bone were de
作者
杨娜
鲍萍萍
雷涛
Yang Na;Bao Pingping;Lei Tao(Department of Stomatology,Tianjin Jinnan District Xianshui Hospital,Tianjin 300350,China;Department of Endodontics,Hospital of Stomatology Affiliated to Nankai University,Tianjin 300041,China)
出处
《中国组织工程研究》
CAS
北大核心
2019年第23期3692-3697,共6页
Chinese Journal of Tissue Engineering Research
关键词
脂联素
糖尿病
牙周炎
RAGE-ROS通路
牙周组织
牙周细胞
凋亡
组织构建
adiponectin
diabetes mellitus
periodontitis
RAGE-ROS signaling pathway
periodontal tissue
periodontal cells
apoptosis
tissue construction
