摘要
目的研究麸炒炮制后枳壳中橙皮苷含量的变化并确定其临床用量。方法含量测定采用HPLC法,使用Agilent 1200高效液相色谱仪,Agilent SB C_(18)色谱柱(4.6×250,5μm),甲醇-0.2%冰醋酸为流动相,梯度洗脱,流速1.0mL·min^(-1),柱温25℃,进样量10μL,检测波长320nm;根据测得麸炒枳壳中柚皮苷含量及相应批次药材含量计算转移率及临床用量。结果 10批麸炒枳壳中,橙皮苷的含量在0.980～1.928mg·g^(-1)范围内,对应转移率分别为45.36～89.06%,即每g饮片相当于1.33g生品。结论本研究所用方法能够准确、高效地分析麸炒枳壳中柚皮苷的含量并计算转移率,临床用量分析结果显示,麸炒炮制后枳壳较生品橙皮苷含量存在显著变化。
OBJECTIVE To investigate variation of hesperidin in Bran fried Fructus aurantii and determine its clinical dosage.METHODS An Agilent 1200 high performance liquid chromatography equipped with Agilent SB C18 column(4.6×250,5μm)was used for content determination,the mobile phase was methanol-0.2%glacial acetic acid in gradient elution,the flow rate was 1.0 mL·min^-1,the column temperature was 25℃,inject volume was 10μL,and the detection wavelength was 320 nm;the obtained content was introduced to calculate transition rate of Bran fried Fructus aurantii with its crude material,and the clinical dosage was finally reckoned.RESULTS In 10 batches of Bran fried Fructus aurantii,the hesperidin content lied in the range of 0.980~1.928 mg·g^-1,with the transition rate between 45.36~89.06%,indicating 1 g of the processed product was equivalent to 1.33 g of crude drug.CONCLUSION The method used was capable of determining hesperidin content in Bran fried Fructus aurantii and calculating transition rate;the results of clinical dosage showed that processing the crude drug brought about significant change in hesperidin content.
作者
文小燕
陈伟英
容丽英
WEN Xiao-yan;CHEN Wei-ying;RONG Li-ying(Department of pharmacy,Second people′s hospital of Zhaoqing,Zhaoqing 526060,China)
出处
《海峡药学》
2019年第4期56-58,共3页
Strait Pharmaceutical Journal
关键词
麸炒枳壳
HPLC
柚皮苷
临床用量
Bran fried Fructus aurantii
HPLC
Hesperidin
Clinical closage
