摘要
通过建立赖氨酸表面修饰的胶体金体系,构建了一种检测霉变花生中黄曲霉毒素B_1(AFB_1)的方法。首先,用柠檬酸三钠还原法制备胶体金(Au NPs),然后在所制备的胶体金溶液中加入赖氨酸,制备了赖氨酸修饰的胶体金溶液(Lys-Au NPs)。利用紫外可见分光光度计和透射电镜对其进行表征,建立了Lys-AuNPs溶液在725 nm和525 nm处吸光度的比值(A_(725)/A_(525))与AFB_1质量浓度之间的相关关系。结果表明:AFB_1质量浓度在1～50 ng/mL范围内与A_(725)/A_(525)具有良好的线性关系,决定系数为0.996,检出限为0.2 ng/mL。花生样品中的加标回收率范围是85%～110%。通过高效液相色谱法(HPLC)对花生中AFB_1质量浓度进行验证,AFB_1质量浓度的预测值与真实值的均方根误差为0.865 1 ng/mL,相关系数为0.996 1。该方法具有快速简单、灵敏度高、操作简便等优点,可运用于霉变花生中AFB_1的快速检测。
Aflatoxin B 1 (AFB 1 ) is the most toxic mycotoxin, which is classified as grade 1 carcinogens by the World Health Organization. A method for detection of AFB 1 in moldy peanut was proposed by using lysine-functionalized gold nanoparticles (Lys AuNPs). AuNPs was synthesized by the citrate reduction method, of which the color was red-wine. To obtain lysine-functionalized AuNPs, lysine solution was added to the prepared AuNPs under stirring by magnetic stirrer. It was characterized by transmission electron microscope and UV-visible spectrophotometry. The detection of AFB 1 was achieved through the competitive reaction mechanism between Hg^2+, AFB 1 and amino acids on lysine surface in the gold nanoparticles solution. As the concentration of AFB 1 in Lys AuNPs solution was increased, the color of the solution was changed from grey to red. UV-visible spectrums were measured to establish the relationship between the A 725 / A 525 value of Lys AuNPs solution and the concentration of AFB 1 . The results showed that there was a good linear relationship in the range of 1~50 ng/mL, and the correlation coefficient was 0.996. The detection limit of AFB 1 was 0.2 ng/mL and the recovery in peanut samples was 85%~110%. In addition, the concentration of AFB 1 in peanut was confirmed by high performance liquid chromatography (HPLC). The method for detection of AFB 1 in moldy peanuts had the advantages of quick simplicity, high sensitivity, and simple operation and so on, which can be applied to the rapid detection of aflatoxin B 1 in moldy peanut and make the assay widely applicable.
作者
黄星奕
叶伟涛
王成全
吕日琴
孙兆燕
HUANG Xingyi;YE Weitao;WANG Chengquan;Lü Riqin;SUN Zhaoyan(School of Food and Biological Engineering,Jiangsu University,Zhenjiang 212013,China)
出处
《农业机械学报》
EI
CAS
CSCD
北大核心
2019年第5期370-375,共6页
Transactions of the Chinese Society for Agricultural Machinery
基金
国家重点研发计划项目(2017YFD0400100)
关键词
花生
黄曲霉毒素B1
胶体金
赖氨酸修饰
peanut
aflatoxin B1
gold nanoparticles
lysine-functionalized
