摘要
目的:研究藤梨根水提取物对人肺癌细胞A549的抑制作用及其机制。方法:用MTT法检测不同剂量的藤梨根水提取物对人肺癌细胞A549的增殖抑制作用。运用免疫印迹法检测各浓度组48hPI3K/Akt信号通路重要蛋白Akt及其磷酸化形式p-Akt的蛋白水平。结果:不同浓度的藤梨根水提取物对人肺癌细胞A549均有抑制作用,且有一定的浓度依赖性。随着浓度升高,p-Akt/Akt值呈降低趋势,差异有统计学意义(P<0.05)。结论:藤梨根水提取物对人肺癌细胞A549增殖起抑制作用,同时与药物浓度有一定的依赖性,其可能通过抑制PI3K/Akt通路激活实现。
Objective:To study the inhibitory effects and mechanism of water extracts from Tengligen on human lung cancer cell A549.Methods:The inhibitory effect of different doses of water extracts from Tengligen on the proliferation of human lung cancer cell line A549 was detected by MTT assay.Western blotting detected the protein levels of Akt and its phosphorylated form of p-Akt in the PI3K/Akt signaling pathway at 48 h in each concentration group.Results:Different concentrations of water extracts from Tengligen had inhibitory effects on human lung cancer cell A549,and there was a concentration-dependent manner.With increasing concentrations,the p-Akt/Akt values showed a decreasing trend (P<0.05).Conclusion:Water extracts from Tengligen can inhibit the proliferation of human lung cancer cell line A549 and have a certain dependence on drug concentration.It may be achieved by inhibiting the activation of PI3K/Akt pathway.
出处
《中医临床研究》
2019年第10期34-36,共3页
Clinical Journal Of Chinese Medicine
关键词
藤梨根提取物
肺癌细胞
抑制
Water extracts form Tengligen
Lung cancer cells
Inhibition
