摘要
为揭示酪氨酸对三色堇花斑形成的分子机制及转录组特征,探究酪氨酸影响三色堇花青素的合成途径,运用转录组学的方法,以清水为对照,采用3 mmol/L酪氨酸处理三色堇非色斑区,并进行转录组测序,获得19 438条表达显著差异基因,其中13 037条上调,6 401条下调。在花青素相关合成途径中,有9个基因差异表达,与花色相关的转录因子中,有1个基因差异表达。结果分析表明酪氨酸处理可以促进三色堇花青素合成基因和调控基因上调,从而促进花色素的形成。为今后三色堇的花色育种以及遗传信息的改良提供了新的思路。
To reveal the molecular mechanism and transcriptome characteristics of pansy floral blotcheseffected by tyrosine and to exploring the effect of tyrosine on anthocyanins biosynthesis of pansy, transcriptome methods,transcriptome sequencing of Viola×wittrockiana non-blotches area with treatments of H2 O or 3 mmol/L tyrosine were performed. 19 438 different expressing genes were obtained by de novo assembly method. 13 037 Unigenes were up-regulated and 6 401 Unigenes were down-regulated. 9 genes differentially expressed in anthocyanin biosynthesis, and 1 anthocyanin-related transcription factor genes differentially expressed. The results showed that tyrosine treatment could promote the up-regulation of anthocyanin biosynthesis genes and regulatory genes thus accelerating anthocyanin formation. provideing a new idea and theoretical basis for pansy to improve color breeding and genetic information in the future.
作者
崔峥
赵安瑾
李雪青
盛玉辉
周扬
赵莹
郭梨锦
王健
Cui Zheng;Zhao Anjin;Li Xueqing;Sheng Yuhui;Zhou Yang;Zhao Ying;Guo Lijin;Wang Jian(Research Center for Terrestrial Biodiversity of the South China Sea,Institute of Tropical Agriculture and Forestry, Hainan University, Haikou, 570228)
出处
《分子植物育种》
CAS
CSCD
北大核心
2019年第8期2458-2466,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(31260488
31760590)资助
关键词
三色堇
花斑
酪氨酸
转录组
Viola×wittrockiana
Floral blotches
Tyrosine
Transcriptome
