摘要
目的探讨右美托咪定在减轻脂多糖(LPS)致大鼠急性肺损伤(ALI)中的作用及相关机制。方法将SD大鼠随机分为:对照组、脂多糖组(LPS组,腹腔注射LPS 5 mg/kg)和右美托咪定+脂多糖组(LD组,腹腔注射右美托咪定25μg/kg+LPS 5 mg/kg)。6 h后,收集左肺支气管肺泡灌洗液(BALF),ELISA检测BALF中白细胞介素-1β(IL-1β),白细胞介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)的含量;取右肺组织进行HE染色观察肺组织病理学变化,并进行肺损伤评分;计算肺组织湿重/干重(W/D)的比值;Western blot检测肺组织中HGMB1、TLR4和TLR2蛋白的表达。结果与对照组相比,LPS组中肺损伤评分、W/D比值、BALF中IL-1β、IL-6、TNF-α的含量明显升高(P<0.05),且肺组织中HMGB1,TLR2,TLR4的表达上调(P<0.05);与LPS组相比,LD组中各指标变化显著减轻(P<0.05)。结论右美托咪定能够减轻LPS致大鼠急性肺损伤,可能与HGMB1和TLRs蛋白表达的抑制有关。
Objective To explore the role and mechanism of demedetomidine in mitigation of lipopolysaccharide(LPS) induced acute lung injury(ALI) in rats. Methods SD rats were randomly divided into control group, lipopolysaccharide group(LPS group, intraperitoneal injection LPS 5 mg/kg), dexmedetomidine+LPS group(LD group and intraperitoneal injection demedetomidine 25 μg/kg and LPS 5 mg/kg). After LPS injection 6 h later, broncho-alveolar lavage fluid(BALF) in left lung was collected for determination of concentrations of interleukin-1β(IL-1β), interleukin-6(IL-6) and tumor necrosis factor(TNF-α) by enzyme linked immunosorbent assay(ELISA). The right lung was removed to observe the pathological changes by HE staining and measure the lung injury score(LIS). The wet to dry lung weight (W/D) ration was calculated. The expression of HGMB1, TLR4 and TLR2 were detected by western blot. Results Compared with control group, the ALI, W/D ration, IL-1β, IL-6 and TNF-α in BALF, and the expression of HMGB1, TLR4 and TLR2 were significantly increased in LPS group( P <0.05). Compared with LPS group, the changes of each index in LD group were significantly relieved ( P <0.05). Conclusions Dexmedetomidine relieves LPS-induced ALI in rats through inhibiting the expression of HGMB1 and TLRs.
作者
韩宏亮
洪道先
陈英
宋俊杰
许远征
庞红利
HAN Hong-liang;HONG Dao-xian;CHEN Ying;SONG Jun-jie;XU Yuan-zheng;PANG Hong-li(Department of Anesthesiology, the First Affiliated Hospital of Henan University, Kaifeng 475000, China)
出处
《基础医学与临床》
CSCD
2019年第5期696-700,共5页
Basic and Clinical Medicine
基金
河南省教育厅科学技术研究项目(18B310010)
