摘要
为高效开发利用魁蚶Scapharca broughtonii加工副产物,以水解度、氨基态氮含量和产物相对分子质量分布为指标,筛选了碱性蛋白酶、风味蛋白酶、酸性蛋白酶、中性蛋白酶、胰蛋白酶5种蛋白酶,通过单因素和响应面试验,建立碱性蛋白酶最佳酶解工艺,并对最优工艺条件下酶解产物的抗氧化效果进行测定。结果表明:从氨基态氮含量和水解度的变化可知,碱性蛋白酶的酶解程度最高,其次为风味蛋白酶和酸性蛋白酶,中性蛋白酶和胰蛋白酶的酶解效果不佳;碱性蛋白酶酶解最佳参数条件为料液比(g∶mL)1∶4、pH 8.5、温度45℃、加酶量800 U/g、酶解时间3 h,在此条件下,水解度(DH)为35.74%;酶解产物具有明显的抗氧化效果,与十分之一用量的VC效果相当。研究表明,碱性蛋白酶是酶解魁蚶加工副产物获得较多小分子蛋白肽的良好用酶,本研究结果可为小肽型水产饲料开发提供基础数据。
The hydrolysis degree, amino nitrogen and molecular weight were investigated in hydrolysates of by-products in processing in burnt-end ark Scapharca broughtonii by five different protease including alkali protease, flavorzyme, acid protease, neutral protease and trypsin by the controlled enzymatic hydrolysis method in order to efficiently develop and utilize the by-products of burnt-end ark and to screen suitable protease. The enzymatic hydrolysis process was optimized by a single factor and response surface experiments, and the resistant oxidation of hydrolysates was detected. The maximal enzymatic hydrolysis degree was found in the hydrolysates of alkaline protease, followed by flavoring protease and acid protease, and the minimal enzymatic hydrolysis in neutral protease and trypsin. The optimum enzymatic hydrolysis of alkaline protease were observed under the conditions of material-liquid ratio=1∶4, pH 8.5, at temperature of 45 ℃, enzyme dose of 800 U/g, and enzymatic hydrolysis for 3 h, with hydrolysis degree(DH) of 35.74%. The hydrolysates had obvious antioxidant effect, equivalent to the 1/10 concentration of Vitamin C. The finding shows that alkaline protease is a good enzyme to obtain more small molecular protein peptides from the processing by-products of burnt-end ark, and that can provide basic research with the development of small peptide type of fishery feed.
作者
刘天红
王颖
李晓
姜晓东
李红艳
孙元芹
纪蕾
LIU Tian-hong;WANG Ying;LI Xiao;JIANG Xiao-dong;LIHong-yan;SUN Yuan-qin;JI Lei(Marine Biology Institute of Shandong Province, Qingdao266104, China)
出处
《大连海洋大学学报》
CAS
CSCD
北大核心
2019年第3期419-427,共9页
Journal of Dalian Ocean University
基金
国家海洋局公益性行业科研专项(201505030-7)
关键词
魁蚶
酶解
氨基态氮
水解度
相对分子质量
Scapharca broughtonii
enzymatic hydrolysis
amino nitrogen
degree of hydrolysis
molecular weight
