摘要
【目的】研究O_(78)血清型大肠杆菌对蛋鸡的致病性,以及单甘油酯对蛋鸡O_(78)血清型大肠杆菌不同致病方式的保护效果,为绿色无抗养鸡提供理论支撑和实践经验。【方法】对福建省某鸡场分离的血清型O_(78)禽源致病性大肠杆菌(APEC),采用口服、颈部皮下和气管内壁接种不同日龄的试验鸡,评价菌株的致病性,建立攻毒模型,然后评估以丙酸单甘油酯、丁酸单甘油酯为主要成分的水溶性液态添加物FraC34对鸡大肠杆菌病的保护效果。【结果】(1)12日龄海兰蛋鸡雄雏口服接种细菌3.0×10~9CFU·羽^(-1),可造成100%发病;但22日龄海兰蛋鸡雄雏分别口服接种细菌3.0×10~9CFU·羽^(-1)和6.0×10~9CFU·羽^(-1)时不发病。12日龄海兰蛋鸡雄雏颈部皮下接种细菌0.4×10~9CFU·羽^(-1),可造成70%的试验鸡发病,22日龄海兰蛋鸡雄雏气管内壁接种细菌1.5×10~9CFU·羽^(-1),可造成25%鸡只发病;(2)在饮水中添加FraC34,早期可改善鸡肠道健康,降低肠道对APEC的易感性,在饮水中添加2～3 L·m^(-3)的FraC34,能够显著降低经由口服途径感染APEC造成的发病率和死亡率,还能够降低经气管内壁接种APEC所造成的死亡率,并且显著降低发病率;当饮水添加3 L·m^(-3)时,可以维持感染鸡的日增重。【结论】鸡O_(78)血清型的APEC对幼雏具有极大的危害性,造成严重经济损失,而中大雏对该菌株具有一定的耐受性;养殖环节添加FraC34,对于鸡感染APEC具有一定的保护作用,并且可以维持鸡的生产性能,可替代部分抗生素的使用,达到较为理想的保护效果。
【Objective】Studying the pathogenicity of serotype O78 Escherichia coli to laying hens with different inoculation methods and the protective effects of single glycerides could provide theoretical support and practical experience for green no-antibiotic chicken breeding.【Method】The virulence of serotype O 78 avian pathogenic Escherichia coli(APEC)strain isolated from a chicken farm in Fujian province was evaluated by oral,subcutaneous and intratracheal inoculation,and the protective effect of water-soluble liquid additive FraC34,composed of butyric and propionic acid monogly cerides,was evaluated in Hy-line laying hens.【Result】The results showed that:(1)With oral inoculation of 3×10^9 CFU bacteria per chicken,the morbidity was 100% for 12-days old Hy-line laying hens,but no disease detected for 22-days old ones with oral inoculation of 3×10^9 or 6×10^9 CFU bacteria per chicken.When the 12-days old chicken were inoculated subcutaneously with 0.4×10^9 CFU bacteria per chicken,the rate of the sick was 70% ,and 25% for 22-days old ones when inoculated intracheally with 1.5×10^9 CFU bacteria per chicken.(2)Adding FraC34 in drinking water of laying hens could improve the intestinal tract and reduce the susceptibility to APEC in the early stage.The addition of 2.0-3.0 L·m^-3 FraC34 in drinking water could significantly reduce the morbidity and mortality rate for chickens infected with APEC by oral inoculation and mortality rate of which with intratracheal inner wall inoculation.The daily gain of chicken could be maintained when treated by 3.0 L·m^-3 FraC34.【Conclusion】The serotype O78 APEC strain was pathogenic to young chicks and led to serious economic losses,but not susceptible for older chicken.FraC34 had protective effect and could maintain the production performance of the chicken infected with APEC,which could be applied instead of some antibiotics for efficient production of poultry.
作者
刘玉涛
凌清标
黄宝钦
吴学敏
王隆柏
周伦江
LIU Yu-tao;LING Qing-biao;HUANG Bao-qin;WU Xue-min;WANG Long-bai;ZHOU Lun-jiang(Institute of Animal Husbandry and Veterinary Medicine,Fujian Academy of AgriculturalSciences,Fuzhou,Fujian 350013,China;Hushi Station of Animal Husbandry andVeterinary Medicine of Xiuyu District,Putian,Fujian 351146,China;Fujian Sunner Development Co.Ltd.,Nanping,Fujian 354100,China)
出处
《福建农业学报》
CAS
CSCD
北大核心
2019年第2期209-213,共5页
Fujian Journal of Agricultural Sciences
基金
福建省科技计划项目--省属公益类科研院所基本科研专项(2017R1023-3)
关键词
禽致病性大肠杆菌
丙/丁酸单甘油酯
海兰蛋鸡
保护效果
avian pathogenic Escherichia coli
butyric/propionic glycerides
Hy-line laying hens
protective effect
