摘要
为明确河南封丘金银花炭疽病病原种类,对采自封丘的金银花炭疽病病叶,依据柯赫氏法则进行分离、纯化、回接和再分离,证明获得的分离菌株是金银花炭疽病的致病菌.对其培养性状、在寄主上的形态特征及萌发方式进行观察,并对其r DNA-ITS序列进行分析,结果表明:致病菌株在PDA培养基上菌落呈白色,后期菌落中央颜色变暗,生分生孢子盘;分生孢子盘具黑色刚毛,分生孢子长圆柱形,两端钝圆,单细胞.核糖体DNA-ITS序列与GenBank数据库胶孢炭疽菌同源性达99%.将封丘金银花炭疽病病原菌鉴定为胶孢炭疽菌Colletotrichum gloeosporioides.
In order to determine the pathogen of Lonicera japonica anthracnose in Henan Province,infected leaves were collected from Fengqiu,Henan Province.The pathogen was isolated,back-inoculated,re-isolated by Koch’s postulates and was identified according to morphological and culture characteristics and the sequence of ribosomal DNA-ITS.The results showed that the fungus colonies on PDA were white,later pale yellow.Acervuli with setae formed in the center of the colonies.Conidia long cylindrical,obtuse at both ends,single-celled.rDNA-ITS sequence analysis indicated that the pathogen and Colletotrichum gloeosporioidesisolate COL4 were 99%in homology.he pathogen was identified as Colletotrichum gloeosporioides.
作者
李志红
翟凤艳
张永霞
王迪
邢登可
鲁海菊
刘英杰
LI Zhihong;ZHAI Fengyan;ZHANG Yongxia;WANG Di;XING Dengke;LU Haiju;LIU Yingjie(School of Resource and Environmental Sciences,Henan Institute of Science and Technology,Xinxiang 453003,China;Department of Life Science and Technology,Honghe College,Mengzi 661199,China)
出处
《河南科技学院学报（自然科学版）》
2019年第2期20-23,共4页
Journal of Henan Institute of Science and Technology(Natural Science Edition)
基金
国家大学生创新训练项目(2018CX29)
河南省高等学校青年骨干教师培养计划项目(2016GGJS109)
关键词
金银花
炭疽病
胶孢炭疽菌
rDNA-ITS序列
鉴定
honeysuckle
anthracnose
Colletotrichum gloeosporioides
rDNA-ITS sequence
idendification
