摘要
以还原性谷胱甘肽(GSH)为主要原料,通过亲核加成反应键合至C_(60)上,合成了一种水溶性富勒烯光敏剂(C_(60)-OH-GSH),并采用UV-Vis、FTIR和DLS对其结构组成进行表征。结果表明:C_(60)-OH-GSH在水溶液中形成了平均粒径约126 nm的纳米颗粒,并且能够稳定存在于多种生理溶液中,具有优异的生物相容性。光动力治疗结果显示,在1μmol/L的浓度下,C_(60)-OH-GSH就能被强度为20 mW/cm^2自然光激发诱导产生大量的活性氧,使4种肿瘤细胞(U87-MG、A549、Hep G2和DU145)活性分别下降至60%～90%;同时,在避光条件下,50μmol/L的C_(60)-OH-GSH对正常细胞没有损伤。
Water-soluble fullerene photosensitizer(C60-OH-GSH) was synthesized by bonding reduced glutathione(GSH) to fullerene C60 via nucleophilic addition reaction. Its chemical composition and structure were characterized by UV-Vis, FTIR and DLS. C60-OH-GSH aggregates to nanoparticles of uniform particle size(hydrodynamic size about 126 nm) in aqueous solution, and it gives high stability in multiple physiological solutions and exhibits excellent biocompatibility. The results of photodynamic therapy show that C60-OH-GSH(1 μmol/L) could be induced to produce a large amount of reactive oxygen species with natural light irradiation at 20 mW/cm^2, making the cell viability of four kinds of tumor cells(U87-MG,A549, HepG2 and DU145) reduce to 60%~ 90%, respectively. At the same time, 50 μmol/L C60-OH-GSH has no damage on normal cells in the dark.
作者
张孝焱
马艺函
张爱清
ZHANG Xiao-yan;MA Yi-han;ZHANG Ai-qing(College of Chemistry & Materials Science, South-Central University for Nationalities, Wuhan 430074, Hubei, China)
出处
《精细化工》
EI
CAS
CSCD
北大核心
2019年第4期582-587,共6页
Fine Chemicals
基金
国家自然科学基金(51502352)~~
关键词
富勒烯
还原性谷胱甘肽
光敏剂
光动力治疗
功能材料
fullerene
reduced glutathione
photosentizer
photodynamic therapy
functional materials
