摘要
目的:建立小鼠全身炎症反应综合征(SIRS)模型,探索紫萁贯众醇提取物中单体成分对羟基苄叉丙酮(4-hydroxybenzylideneacetone,HBAc),3,4-二羟基苄叉丙酮(3,4-dihydroxybenzylideneacetone,DHBAc)对SIRS模型小鼠的保护作用及机制。方法:BALB/c小鼠随机分为正常组,模型组,HBAc,DHBAc低、中、高剂量(25,50,100μg·kg^(-1))组。预防给药7 d后腹腔注射脂多糖(LPS),造模5 h后检测小鼠肛温、呼吸频率、白细胞、血小板计数、白细胞分类、糖脂代谢以及肺组织炎症因子和炎症相关蛋白磷酸化情况。结果:与正常组比较,模型组小鼠腹腔注射LPS(6 mg·kg^(-1))可致小鼠呼吸频率降低(P<0.05),体温明显降低(P<0.01),外周血白细胞数和单核细胞百分比增加(P<0.01),血小板减少(P<0.01),血糖水平降低(P<0.05),肺组织中白细胞介素-1β分泌增多(P<0.01)。与模型组比较,HBAc,DHBAc均明显增加动物呼吸频率,升高动物体温,降低外周白细胞水平以及单核细胞百分比(P<0.05,P<0.01),并显著升高血糖水平(P<0.05,P<0.01),减少肺组织中白细胞介素-1β的分泌(P<0.01)。结论:腹腔注射LPS致小鼠SIRS模型成立,HBAc,DHBAc对LPS致小鼠SIRS模型有一定的保护作用,可能通过IκB,c-JUN通路发挥抗炎作用。
Objective: To investigate the protective effect of phenolic compounds 4-hydroxybenzylideneacetone and( HBAc) 3,4-dihydroxybenzylideneacetone( DHBAc) of Osmundae Rhizoma on the systemic inflammatory response syndrome( SIRS) in mice by establishing the mice model of SIRS. Method:BALB/c mice were randomly divided into the normal group,the SIRS model group and the different doses of HBAC and DHBAc group( 25,50,100 μg·kg^-1). Lipopolysaccharides( LPS) was injected intraperitoneally after 7 days of prophylactic administration. After 5 hours of modeling,the anus temperature,respiratory rate,the number of white blood cell( WBC) and platelets( PLT),WBC classification,glycolipid metabolism,inflammatory factor and signal transducing phosphorylated protein of lung were measured. Result: Intraperitoneal injection with LPS( 6 mg·kg^-1) in mice can significantly reduce the respiratory rate( P <0. 05) and the body temperature( P< 0. 01),decrease the number of WBC,PLT( P< 0. 01,P <0. 01) and blood glucose( P< 0. 05),and increase the secretion of interleukin-1β( P< 0. 01). HBAC and DHBAC significantly increased the respiratory rate( P< 0. 05,P <0. 01) and the body temperature( P <0. 05,P< 0. 01) of mice,decreased MONO( P< 0. 05,P <0. 01),and significantly increased the level of blood glucose( P< 0. 05,P <0. 01),and decreased the secretion of interleukin-1β( P< 0. 01). Conclusion: The SIRS model can be established through intraperitoneal injection of LPS. HBAc and DHBAc have protective effects on endotoxin-induced SIRS in mice, and may exert antiinflammatory effects through IκB and c-JUN pathways.
作者
李凯
杨庆
周恬恬
闫思超
翁小刚
杨岚
陈颖
王娅杰
姜晓慧
郭媛
朱晓新
李玉洁
LI Kai;YANG Qing;ZHOU Tian-tian;YAN Si-chao;WENG Xiao-gang;YANG Lan;CHEN Ying;WANG Ya-jie;JIANG Xiao-hui;GUO Yuan;ZHU Xiao-xin;LI Yu-jie(Anhui University of Chinese Medicine, Hefei 230038,China;Research Center of Artemisinin,China Academy of Chinese Medical Sciences ,Beijing 100700,China;Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences ,Beijing 100700,China)
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2019年第9期55-60,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
科技部中捷科技合作项目(2017-42-4)
国家自然科学基金面上项目(81673640)
"重大新药创制"科技重大专项(2017ZX09101002-002-008)
关键词
紫萁贯众
全身炎症反应综合征
内毒素
Osmundae Rhizoma
systemic inflammatory response syndrome
endotoxin
