摘要
目的选育抗生素G-418高产的棘孢小单孢菌突变株,以提高抗生素G-418的产量。方法采用紫外诱变、NTG诱变、微波诱变、超声诱变的方法对棘孢小单孢菌进行诱变处理,结合生物活性测定方法,以发酵单位为主要评价标准进行筛选。结果获得两株G-418高产菌株,其产量比出发菌株分别提高了102%和115%。结论本研究表明紫外诱变可以显著提高G-418产生菌的正突变率,尤其是种子液衰亡期的正突变率最高,为选育G-418棘孢小单孢高产菌株奠定了基础。
Objective The purpose of the study is to breed the mutanted Micromonospora echinoapora which can increase the antibiotic production of G-418. Methods By means of the mutagenesis of UV, NTG, microwave, and ultrasonic, combined with the bioactivity assay, the high-yield mutants were selected based on the titre of G-418. Results Two high-yielding strains were obtained with G-418 production increased by 102% and 115%, respectively, compared to the original strain. Conclusion This study shows that UV mutagenesis can significantly increase the positive mutation rate of G-418 producing strains, and especially the seed broth in the decline phase had the highest positive mutation rate, which lays a foundation for the breeding of G-418 high-yielding strains of Micromonospora.
作者
钟艾玲
田敏
刘艳全
易欣
龙燕
雷叶明
王富强
刘亚洲
Zhong Ai-ling;Tian Min;Liu Yan-quan;Yi Xin;Long Yan;Lei Ye-ming;Wang Fu-qiang;Liu Ya-zhou(Antibiotics Research and Re-evaluation Key Laboratory of Sichuan Province, Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu 610052;National Engineering Research Center for Microbial Drug Biosynthesis, Chengdu Yacht Bio-Technology Co., Ltd., Chengdu 610041)
出处
《中国抗生素杂志》
CAS
CSCD
2019年第4期422-425,共4页
Chinese Journal of Antibiotics
基金
四川省科技计划应用基础研究项目(No.2017JY0256)
四川省医药微生物共享服务平台建设(No.2018TJPT004)
关键词
诱变方法
衰亡期
选育
Mutation methods
Decline phase
Breeding
