摘要
从实验室现存益生菌菌库中选取八株乳杆菌为出发菌株,对菌株的胆固醇降解能力、自由基清除能力、γ-氨基丁酸产生能力及人工胃肠液耐受能力进行体外益生效果评价。结果表明,约氏乳杆菌Lactobacillusyoelii142不仅胆固醇降解能力达到53.9%、DPPH抗氧化能力达到79.5%,6h后还能在人工胃液和肠液中保持较高存活率(存活率分别为86.7%和90%)。因此,L.yoelii142具有良好的潜在益生功能,为高活性直投式发酵剂菌株的筛选、应用及功能产品的开发提供重要理论指导。
Eight Lactobacillus strains were selected from the probiotic bacteria pools in the laboratory as the starting strains,and the in vitro probiotic effects of these strains on cholesterol degradation,free radical scavenging ability,γ-aminobutyric acid producing ability and artificial gastrointestinal fluid tolerance were studied.The results show that Lactobacillus yoelii 142(L.yoelii 142)not only has a cholesterol degradation of 53.9%and a DPPH antioxidant capacity of 79.5%.The survival rate of artificial gastrointestinal fluid is 86.7%(gastric)and 90%(intestinal)respectlvely after 6h.It could be drawn as a conclusion that L.yoelii 142 has good potential probiotic function,which provides important theoretical guidance for the screening,application and development of functional products of high-activity direct-injection starter strains.
作者
赵鑫君
杨志鹏
张晶晶
王婷
ZHAO Xin-jun;YANG Zhi-peng;ZHANG Jing-jing;WANG Ting(School of Biological Engineering,Qilu University of Technology(Shandong Academy of Sciences),Jinan 250353,China)
出处
《齐鲁工业大学学报》
2019年第2期32-36,共5页
Journal of Qilu University of Technology
基金
国家自然科学基金青年基金(31701576)
中国博士后科学基金第62批面上资助项目(2017M622110)
山东省博士后创新项目专项资助(201703081)
关键词
乳杆菌
降解胆固醇
Γ-氨基丁酸
抗氧化
人工胃肠液耐受能力
益生评价
bacillus lactis
cholesterol degradation
γ-aminobutyrate
antioxidation
artificial gastrointestinal fluid tolerance
probiotics evaluation
