摘要
采用脂质体技术制备乙酰胆碱酯酶的微反应器,并以微反应器、壳聚糖和二氧化硅为基质,构建以多层纳米酶膜修饰电极为核心的有机磷农药残留快速检测酶电化学生物传感器。结果表明,酶脂质体微反应器平均粒径为(7.26±0.75)μm;修饰电极多层酶膜的最佳层数为6个双层;以敌敌畏作为有机磷农药模型,分别在其浓度为0.25～1.75μmol/L和2.00～10.00μmol/L线性范围内,传感器的峰电流值与乙酰胆碱酯酶抑制率呈现出良好的线性关系,回归方程分别为I=28.58C+5.35和I=2.38C+51.25;最低检出限为(0.72±0.065)μg/L(信噪比为3);此新型酶纳米生物传感器具有良好的灵敏度、重复性和选择性。
Acetylcholinesterase (AChE) liposomes bioreactor (ALB), prepared by encapsulating the enzyme AChE into L-α-phosphatidylcholine liposomes, was spherical in shape. A novel AChE biosensor based on multilayer films containing SiO 2 , chitosan (CS) and ALB was developed. The results showed that ALB had a mean diameter of (7.26 ± 0.75)?m. The biosensor based on six bilayers of multilayer films was the best. The properties of the biosensor were evaluated by electrochemical measurements. Based on the inhibition of organophosphate pesticides on the AChE activity, by using dichlorvos as a model organophosphorus compound, we observed that the inhibition of dichlorvos was proportional to its concentration within the range from 0.25 to 1.75 μmol/L and from 2.00 to 10.00 μmol/L, and the corresponding equations were I = 28.58C + 5.35 and I = 2.38C + 51.25, with a detection limit (LOD) of (0.72 ± 0.065)μg/L estimated at a signal-to-noise ratio of 3. The fabrication of the biosensor was simple, with fast response and acceptable selectivity.
作者
关桦楠
韩博林
龚德状
阎秀峰
GUAN Huanan;HAN Bolin;GONG Dezhuang;YAN Xiufeng(Alkali Soil Natural Environmental Science Center, Northeast Forestry University, Harbin 150040, China;College of Food Engineering, Harbin University of Commerce, Harbin 150076, China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2019年第8期280-286,共7页
Food Science
基金
国家自然科学基金面上项目(31370649)
国家自然科学基金青年科学基金项目(31201376)
黑龙江省自然科学基金项目(C2016034)
黑龙江省普通本科高等学校青年创新人新才培养计划项目(UNPYSCT-2016060)
中国博士后基金资助项目(2014T70304
2013M531009)
黑龙江省博士后资助项目(LBH-Z13002)
哈尔滨商业大学科研项目(17XN026)
哈尔滨商业大学研究生创新科研资金项目(YJSCX2017-462HSD
YJSCX2018-538HSD)
