摘要
为确定患乳房炎奶牛乳中金黄色葡萄球菌的流行性和耐药性状况,试验自2018年4月至2018年5月采集了新疆地区70例和内蒙古地区50例患乳房炎奶牛生鲜乳样。通过对奶样中金黄色葡萄球菌进行增菌培养、分离纯化,KB纸片扩散法药敏试验及PCR耐药基因检测发现,新疆和内蒙古地区奶样中金黄色葡萄球菌分离率分别为28.6%和54.0%;新疆地区分离的金黄色葡萄球菌菌株对青霉素G的耐药性最高(75.0%),其次是磺胺异噁唑(60.0%)、林可霉素(55.0%);内蒙古地区的金黄色葡萄球菌菌株同样对青霉素G高度耐受,耐药率为70.4%,其次为林可霉素(63.0%)、克林霉素(51.9%);两地区所分离菌株中,88.9%以上携带多种耐药基因,呈现多重耐药性;两地区所检出的高频耐药基因分别是新疆地区的ermA(30.0%)、dfrS1(30.0%)基因和内蒙古地区的lnuA(46.2%)基因。
To evaluate the epidemicity and antimicrobial resistance of Staphylococcus aureus in the milk of mastitis cows,70 samples from Xinjiang and 50 samples from Inner Mongolia were collected from April to May in 2018.Staphylococcus aureus was cultivated,separated and purified.Antibiotic susceptibility test of purified isolates with KB paper and PCR analysis for the drug resistance gene were carried out.The results showed that the separation rate of Staphylococcus aureus in Xinjiang and Inner Mongolia was 28.6%and 54.0%,respectively.Penicillin G(75.0%)was the highest resistant antibiotic for Staphylococcus aureus isolated from Xinjiang,followed by sulfis oxazole(60.0%),lincomycin(55.0%).For the isolates from Inner Mongolia,penicillin G(70.4%)was also the highest resistant drug,followed by lincomycin(63.0%)and clindamycin(51.9%).The results of the resistance gene test showed that multi-resistance genes were detected in more than 88.9%of the isolates.The highest frequency resistance genes of isolated were ermA(30.0%)and dfrS 1(30.0%)in Xinjiang and lnuA(46.2%)in Inner Mongolia,respectively.
作者
于忠娜
贾凌云
甄天元
王军
韩荣伟
YU Zhongna;JIA Lingyun;ZHEN Tianyuan;WANG Jun;HAN Rongwei(Haidu College,Qingdao Agricultural University,Laiyang 265200,China;College of Food Science and Engineering,Qingdao Agricultural University,Qingdao 266109,China)
出处
《中国畜牧兽医》
CAS
北大核心
2019年第4期1199-1208,共10页
China Animal Husbandry & Veterinary Medicine
基金
山东省高等学校科学技术计划项目(J17KA131)
农业农村部公益性农业行业科研专项(201403071-5)
国家奶产品质量安全风险评估重大专项(GJFP201800804)
山东省自然基金青年基金项目(2015ZRB01095)
山东省重点研发计划项目(2016GSF120010)
山东省优秀中青年山东省高等学校科技计划项目科学家科研奖励基金项目(BS2014NY011)
青岛农业大学高层次人才科研基金项目(6631115043)
关键词
乳房炎
金黄色葡萄球菌
生鲜乳
流行性
耐药性
mastitis
Staphylococcus aureus
raw milk
epidemicity
antimicrobial resistance
