摘要
光动力疗法(PDT)是光敏剂通过光激活产生的单线态氧(~1O_2)来杀死癌细胞。光动力疗法的3个关键因素主要有:光、光敏剂与组织氧。在PDT治疗过程中,氧气消耗和实体瘤中固有的缺氧微环境可能进一步导致供氧不足,阻碍光动力疗效,而光热疗法(PTT)不受乏氧环境的影响,且具有无创性、低毒性等优点。将全氟化碳(PFCs)和光敏剂(Pba)接枝到透明质酸(HA)链中,然后负载聚多巴胺纳米粒子(PDANPs),设计出了一种新型的靶向自供氧光动力与光热协同治疗体系。由于PDANPs优良的光热转换性能、PFC较高的氧亲和力和HA的肿瘤靶向性,使得该协同疗法的抗肿瘤作用显著提高,细胞毒性实验以及细胞摄取实验证明了其增强的光热与光动力治疗效果。
With the increasing of morbidity and mortality,cancer has become one of the leading causes of death worldwide.Photodynamic therapy (PDT) kills cancer cells by using reactive singlet oxygen (1O2) which is produced from photosensitizer by light activation,and has been widely utilized for many localized and superficial cancer treatments over the past four decades.The three major components of PDT are light,photosensitizer,and tissue oxygen.In PDT treatment,oxygen depletion and inherent hypoxia microenvironment in solid tumors may lead to further hypoxia,which in turn can hinder PDT efficacy.However,photothermal therapy (PTT) is not affected by the hypoxic environment,and has gained widespread attention owing to its noninvasive and low toxicity.Polydopamine nanoparticles (PDANPs) were utilized as a PTT agent for tumor treatment because of its good biocompatibility and high photothermal conversion efficiency.Here,we created a PDT-PTT system by grafting perfluorocarbon and photosensitizer into the hyaluronic acid (HA) chains and loading the PDANPs (Ac-HA-PFC-Pba-PDANPs).This PDTPTT system consisted of a PDANPs core and a fluorinated HA photosensitizer shell.The synthesized Ac-HA-PFC-Pba-PDANPs had a relatively narrow particle size distribution (around 160 nm),and can be easily endocytosed by cancer cells.Furthermore,an efficient photothermal conversion function for PTT was also shown in the Ac-HA-PFC-Pba-PDANPs system.It raised up to 45 ℃ to kill cancer cells in a short time.With the excellent photothermal conversion ability of PDANPs,the high oxygen affinity of perfluorocarbon segments and the tumor targeting of HA,this system provides superior therapeutic efficacy against cancer cells.The anti-tumor effect was significantly improved,which was confirmed by cytotoxicity test and cell uptake studies.This novel nanoparticle provides a promising approach for synergetic PDT-PTT of hypoxic tumors.
作者
田君
张伟安
TIAN Jun;ZHANG Weian(School of Chemistry and Molecular Engineering,East China University of Science and Technology,Shanghai 200237,China)
出处
《华东理工大学学报（自然科学版）》
CAS
CSCD
北大核心
2019年第2期285-292,共8页
Journal of East China University of Science and Technology
关键词
自供氧
全氟化碳
聚多巴胺
光动力治疗
光热治疗
oxygen self-carrying
perfluocarbon
polydopamine
photodynamic therapy
photothermal therapy
