摘要
旨在研究日粮添加油脂和不同出生类型对羔羊肌肉胶原蛋白特性及相关基因表达的影响。本试验选取64只2月龄断奶双羔(twin born,TW;32只)和三羔(triplet born,TR;32只)湖羊公羔,分别饲喂基础日粮(control, CON)和添加2%大豆油(soybean oil,SBO)的日粮,采用2×2析因试验设计,分为4个处理(TW-CON,TW-SBO,TR-CON,TR-SBO),每个处理16个重复,每个重复1只羊。90 d后屠宰采集羔羊背最长肌,测定其胶原蛋白含量、相关酶活以及肌肉发育相关基因表达水平等。结果表明:1) TW组的Ⅰ型胶原蛋白含量显著高于TR组(P<0.05)。日粮添加油脂有提高可溶性胶原蛋白含量的趋势(0.05<P<0.10),但对胶原蛋白溶解率无显著性影响(P>0.05)。2)基质金属蛋白酶13(MMP-13)含量因日粮中油脂添加而显著下降(P<0.05),而吡啶诺林(PYD)和基质金属蛋白酶抑制剂(TIMP-1)则受日粮油脂添加和出生类型交互作用的显著影响(P<0.05)。3)MYOD1在双羔中表达水平显著高于三羔(P<0.05)。日粮油脂添加显著降低了FN1的相对表达水平(P<0.05),而FGF2和COL3A1的相对表达水平则表现出受日粮油脂添加和出生类型交互作用影响的趋势(0.05<P<0.10),其它基因如FGFR1、TGFβ1、COL1A1等则不受日粮油脂和出生类型的显著影响(P>0.05)。结果提示,双羔肌肉中胶原蛋白含量高于三羔,油脂有促进胶原蛋白含量增加的趋势,且日粮油脂和出生类型共同作用胶原蛋白代谢相关酶含量及基因的表达,进而影响肌肉中胶原蛋白的含量。
This study aimed to investigate the effects of dietary soybean oil supplementation and different birth types on collagen characteristics and related genes expression in muscle of lamb.Sixty-four male Hu lambs with twin born(TW,32 lambs)and triplet born(TR,32 lambs)types weaned at 2 months old were selected and fed basal diet(CON)or diet with 2%soybean oil supplemented(SBO).Lambs were randomly divided into 4 treatments(TW-CON,TW-SBO,TR-CON,TR-SBO)according to 2×2 factorial experimental design,each treatment had 16 biological replication,and 1 lamb in each replication.Lambs were slaughtered after 90 days of feeding,and longissimus dorsi muscle samples were collected for detecting the content of collagen,enzymes activity and muscle development-related genes expression level.The results indicated that:1)The collagen typeⅠcontent in TW groups was significantly higher than that in TR groups(P<0.05).Dietary soybean oil supplementation increased collagen content(0.05<P<0.10),while had no significant effect on collagen solubility(P>0.05).2)Matrix metalloproteinases-13(MMP-13)content decreased significantly with diet soybean oil supplementation(P<0.05),while pyridinoline(PYD)and tissue inhibitor of metalloproteinases(TIMP-1)were affected significantly by the interaction effects between dietary soybean oil supplementation and birth types(P<0.05).3)MYOD 1 expression level was significantly higher in TW groups than that in TR groups(P<0.05).The expression level of FN 1 decreased significantly with soybean oil supplemented in diet(P<0.05),whereas FGF 2 and COL 3 A 1 expression levels were affected by the interaction effects between dietary soybean oil supplementation and birth types(0.05<P<0.10).In addition,the expression levels of FGFR 1,TGFβ1 and COL 1 A 1 were not significantly affected by the soybean oil supplementation or birth types(P>0.05).In conclusion,twin born lambs contain more collagen in muscle than triplet born lambs,and soybean oil supplementation show a trend to increase the collagen content in muscle.Moreo
作者
王波
刁志成
马勇
李发弟
曲扬华
徐晨晨
卢晓楠
罗海玲
WANG Bo;DIAO Zhicheng;MA Yong;LI Fadi;QU Yanghua;XU Chenchen;LU Xiaonan;LUO Hailing(State Key Laboratory of Animal Nutrition,College of Animal Science and Technology,China Agricultural University,Beijing 100193,China;College of Pastoral Agricultural Science and Technology,Lanzhou University,Lanzhou 730020,China)
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2019年第4期771-780,共10页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
国家重点研发计划(2018YFD0500402)
国家肉羊产业技术体系(CARS-38)
