摘要
为了解决耐盐石油降解菌生物量不足的问题,以耐盐石油降解菌HDMP2为研究对象,鉴定其菌系归属,并采用单因素分析、因子分析和主成分分析方法分析其生长因子。结果表明,HDMP2菌株的5d柴油降解率为27.49%,通过BLAST比对和发育树分析,鉴定其为Halomonas sp.nmyj-1;当盐含量为5%、pH值为7.07、转速为160r·min^(-1)、接种量为2.5%、油含量为0.4%、培养时间为3d时,HDMP2菌株的生物量最高;6个生长因子对HDMP2菌株生物量的影响大小依次为转速、接种量、pH值、盐含量、油含量和培养时间。
In order to solve the problem of insufficient biomass of salt-tolerant petroleum degrading bacteria,using salt-tolerant petroleum degrading bacterium HDMP2 as a research object,we identified its classification and analyzed its growth factors by single-factor analysis,factor analysis,and principal component analysis.The results indicate that the diesel degradation rate of strain HDMP2 is 27.49% in 5 days,and it is identified as Halomonas sp.nmyj-1 by BLAST alignment and phylogenetic tree analysis.Moreover,the biomass of strain HDMP2 is the highest under the conditions as follows:the salt content of 5%,the pH value of 7.07,the rotation speed of 160 r·min……-1 ,the inoculation amount of 2.5%,the oil content of 0.4%,and the culture time of 3 d .Furthermore,the influence degree of 6 growth factors on the biomass of strain HDMP2 is in a following sequential order from the highest to the lowest:rotation speed,inoculation amount,pH value,salt content,oil content,and culture time.
作者
刘营营
史可
顾蒙
薛建良
LIU Yingying;SHI Ke;GU Meng;XUE Jianliang(College of Chemical and Environmental Engineering,Shandong University of Science and Technology,Qingdao 266590,China)
出处
《化学与生物工程》
CAS
2019年第4期1-5,共5页
Chemistry & Bioengineering
基金
国家自然科学基金项目(51408347)
关键词
耐盐石油降解菌
生长因子
主成分分析
salt-tolerant petroleum degrading bacterium
growth factor
principal component analysis
