摘要
目的建立稳定的减体积肝移植排斥模型。方法肝移植供鼠为雄性Lewis大鼠,8~10周龄,体重为210~250 g;受鼠为雄性BN大鼠,8~10周龄,体重为210~250 g,供受鼠体重相差不超过10 g。实验分为三组:原位移植组(n=65);50%减体积(保留右上叶和中叶)肝移植组(n=65);30%减体积(保留右叶和尾状叶)肝移植组(n=65)。观察供肝切取时间、供肝修整时间、无肝期时间、肝脏冷缺血时间、受鼠手术时间等手术相关指标;观察比较受鼠术后一般状况及存活时间、术后外周血清肝功能指标的变化以及术后肝组织病理改变。结果三组手术相关指标无明显差异;30%组1、3、7和14 d存活率与原位组及50%组相比,明显降低,差异有统计学意义(P<0.05),50%组与原位组相比较,差异无统计学意义(P>0.05);原位、50%和30%组ALT、AST水平均是第1天最高,第3天降到最低,后又有所升高,在第5天后又开始降低,30%组各时间点与原位、50%组相比较,明显升高,差异均有统计学意义(P<0.05),50%组与原位组相比较,差异无统计学意义(P>0.05);TBIL水平则从术后1 d开始缓慢升高,至第5天时急剧升高,直到14 d;30%组术后5、7和14 d与原位、50%组相比较,明显升高,差异均有统计学意义(P<0.05),50%组与原位组相比较,差异无统计学意义(P>0.05);原位、50%和30%组均为排斥模型,各组排斥活动指数评分随着时间推迟均逐渐升高。结论选择大鼠Lewis→BN建立肝移植排斥模型,并通过大鼠原位、50%减体积、30%减体积肝移植大鼠术后存活率及病理比较,最终选定50%减体积肝移植排斥模型作为后续实验研究模型。
Objective To establish a stable model of reduced-size liver transplantations with rejection in rats. Methods Inbred adult male Lewis rats (weighing 220-250 g, 8-10 weeks old) served as donors in liver transplantation, and inbred adult male Brown Norway (BN) rats (weighing 220-250 g, 8-10 weeks old) served as recipients. Orthotopic group, 50% reduced-size liver transplantation rejection group (50% group) and 30% reduced-size liver transplantation rejection group (30% group) were developed. The 1-, 3-, 7- and 14-survival rate was observed. Besides, the levels of ALT, AST and TBIL in the peripheral blood plasma were determined. The liver tissues were obtained, and HE staining was used to examine the pathological changes and rejection severity of the liver specimens. Results No significant difference in the operation-related indicators was found among the three groups. The 1-, 3-, 7- and 14-day survival rate in orthotopic group was as follows: 91.20%, 86.50%, 81.10% and 75.70%;85.00%, 80.00%, 62.50% and 45.00%;65.00%, 50.00%, 35.00% and 17.50%, respectively. The 1-, 3-, 7- and 14-survival rate in 30% group was significantly lower than in other groups (P<0.05), and showed no statistical difference between 50% group and orthotopic group (P>0.05). The ALT, AST and TBIL levels were significantly higher in 30% group than in control group at all time points (P<0.05), but there was no statistical difference between 50% group and orthotopic group (P>0.05). All groups were the models of liver transplantations with rejection in rats, and rejection activity index score was increased with time delay. Conclusion Lewis→BN rats are chosen to establish liver transplant rejection model, and by comparing the survival rate and pathological changes among the groups, finally 50% group is selected to be the model for a follow-up study.
作者
吴斌
高伟
董冲
杨洋
尹明丽
宋红丽
沈中阳
Wu Bin;Gao Wei;Dong Chong;Yang Yang;Yin Mingli;Song Hongli;Shen Zhongyang(Organ Transplantation Center, Tianjin First Center Hospital,Tianjin Clinical Research Center for Organ Transplantation,Tianjin Key Laboratory for Organ Transplantation, Tianjin 300192, China)
出处
《中华器官移植杂志》
CAS
CSCD
北大核心
2018年第10期620-626,共7页
Chinese Journal of Organ Transplantation
关键词
肝移植
排斥
大鼠
Liver transplantation
Rejection
Rats
