摘要
目的比较3种不同方法小体积大鼠肝移植模型建立的途径。方法选取体重相近的雄性Sprague-Dawley大鼠作为供受体,分别采用体内切除肝脏灌注、体内灌注后原位切除肝脏和体外切除肝脏的方法获取小体积供肝,采用Kamada两套管法植入供肝。比较3种方法的术后1周存活率及并发症发生率。结果体内切除肝脏灌注组、体内灌注后原位切除肝脏组、体外切除肝脏组的无肝期、冷缺血及总手术时间的差异均无统计学意义(P值均>0.05);术后1周存活率分别为80.0%、75.0%、82.5%,差异均无统计学意义(P值均>0.05);术后并发症发生率分别为20.0%、25.0%、17.5%,差异均无统计学意义(P值均>0.05)。结论不同的获取移植物的方法都可以保证较高的术后存活率,可根据具体实验需要进行选择。
Objective To compare three methods in establishing small-for-size liver transplantation model in rats.Methods Male Sprague-Dawley rats with similar body weights were selected as the donors and recipients.The small-for-size grafts were harvested by resection of partial liver lobes using 3 different methods.Hepatectomy was performed in situ before graft irrigation in the first group and after graft irrigation in the second group;in the third group liver lobes were removed on back-table after whole liver irrigation.The grafts were implanted according to Kamada＇s method.The survival rates and complication frequencies were compared between the three groups one week later.Results There were no significant differences in the anhepatic phase,cold ischemia and total operation periods between the three groups.The one week survival rates in the first,second and third groups were 80.0%,75.0%,and 82.5%（P〉0.05）,and the complication frequencies were 20.0%,25.0%,and 17.5%（P〉0.05）,respectively.Conclusion All the three methods can achieve a satisfactory survival of the animals,and they may be choosen according to the need of experiment.（Shanghai Med J,2010,33： 576-578）
出处
《上海医学》
CAS
CSCD
北大核心
2010年第6期576-578,共3页
Shanghai Medical Journal
基金
上海市科学技术委员会<小体积肝移植术后胆道并发症的预防及干预研究>课题(09411952100)资助
关键词
肝移植
大鼠
动物模型
小体积
Liver transplantation
Rat
Animal model
Small-for-size
