摘要
本文研究碳源调控磷回收强化生物脱氮除磷(biological bio-nutrient removal-carbon regulation and phosphorus recovery,BBNR-CPR)反应器耐低温特性.不断降低BBNR-CPR反应器运行温度,发现BBNR-CPR反应器能够长期在低温(≤15℃)、低C/N比(<4. 16)条件下稳定运行,维持总磷的平均去除率为91. 20%,氨氮的平均去除率为81. 10%,总氮的平均去除率为58. 62%.随着运行时间的增加与温度的下降,BBNR-CPR反应器生物内膜具有脱氮除磷以及PHA贮存功能的种属Candidatus_Competibacter、Candidatus_Accumulibacter、Run-SP154、Thauera、Candidatus_Nitrotoga的相对丰度不断提高,成为耐受低温条件的优势种属.在相同碳源浓度、合成PHA的时间一致的条件下,生物膜内PHA的合成量受温度影响; 25、15和8℃合成的PHA量分别占生物膜干重的16. 24%、11. 49%和9. 01%.预贮存PHA的生物膜具有耐受低温的能力;在高PHA水平,8℃和15℃的除磷效率分别为97. 46%和100%,脱氮效率分别为55. 15%和82. 55%;而在低PHA水平,8℃和15℃的除磷效率分别为11. 39%和35. 02%,脱氮效率分别为0%和12. 10%.
This study investigated the removal of nitrogen and phosphorous by a biological bio-nutrient removal-carbon regulation and phosphorus recovery (BBNR-CPR) reactor at low temperature. The operating temperature of the BBNR-CPR reactor was continuously reduced, and it was found that the BBNR-CPR reactor could operate steadily at low temperature (<15℃) and low C/N ratio (<4.16). The average removal rates of total phosphorus, ammonia, and total nitrogen were 91.20%, 81.10%, and 58.62%, respectively. With increasing running time and decreasing temperature, the relative abundance of Candidatus_Competibacter, Candidatus_Accumulibacter, Run-SP 154, Thauera, and Candidatus_Nitrotoga increased. These bacteria had the functions of nitrogen and phosphorus removal and the storage of polyhydroxyalkanoate (PHA) in the biofilm of the BBNR-CPR reactor and became the dominant species to tolerate low temperature. It was found that low temperatures reduced the amount of PHA synthesized for a given equal carbon source concentration and reaction time. The amounts of PHA synthesized at 25℃, 15℃, and 8℃ accounted for 16.24%, 11.49%, and 9.01% of the dry weight of the biofilm, respectively. The pre-stored PHA biofilm has the capacity to resist low temperature. At high PHA levels, the phosphorus removal efficiencies at 8℃ and 15℃ were 97.46% and 100%, respectively, and the denitrification efficiencies were 55.15% and 82.55%. At low PHA levels, the phosphorus removal efficiencies at 8℃ and 15℃ were 11.39% and 35.02%, respectively, and the denitrification efficiencies were 0% and 12.10%, respectively.
作者
杨建鹏
张健
田晴
朱艳彬
李方
Wolfgang Sand
YANG Jian-peng;ZHANG Jian;TIAN Qing;ZHU Yan-bin;LI Fang(College of Environmental Science and Engineering, Donghua University, Shanghai 201620,China)
出处
《环境科学》
EI
CAS
CSCD
北大核心
2019年第4期1914-1921,共8页
Environmental Science
基金
国家自然科学基金项目(51478099)
国家重点研发计划项目(2016YFC0400501)
上海市自然科学基金项目(16ZR1402000)
关键词
脱氮除磷
聚羟基烷酸酯(PHA)
低温
低C/N
混合菌群
nitrogen and phosphorus removal
polyhydroxyalkanoate (PHA)
low temperature low C/N
mixed growth microbial community
