摘要
[目的]分析不同急性低温胁迫强度、胁迫持续时间下条纹锯鮨血清生化指标的变化,为其健康养殖和耐寒机制研究提供理论依据。[方法]以条纹锯鮨为研究对象,分别从暂养水温13℃急性胁迫到3、5、7℃,分析3个急性低温胁迫强度、不同胁迫时间(0、2、6、12、24、48 h)对条纹锯鮨血清生化指标的影响。[结果]Ⅰ、Ⅱ组谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)和肌酸激酶同工酶(CK-MB)活性呈先升后降的趋势,各组碱性磷酸酶(ALP)活性均显著高于胁迫前(P<0.05);Ⅰ、Ⅱ组血清总胆固醇(TC)和甘油三酯(TG)先降后升至胁迫前水平,12 h后Ⅲ组总蛋白(TP)、TG、TC含量均显著低于胁迫前水平(P<0.05),Ⅰ、Ⅱ组葡萄糖(GLU)含量48 h时显著上升(P<0.05);Ⅰ、Ⅱ组48 h时血清离子与胁迫前无显著差异(P>0.05),Ⅲ组K^+和Ca^(2+)含量显著下降(P<0.05),Na^+和Cl^-含量显著上升(P<0.05)。[结论]在条纹锯鮨养殖生产和低温驯养过程中,应尽量降低胁迫强度和缩短胁迫时间。
[Objective] To analyze the changes of serum biochemical parameters in Centropristis striata under different acute low temperature stress and the duration of the stress and provide theoretical basis for healthy breeding and cold-resistant mechanism of C. striata .[Method] Using C. striata as research subject, the Effects of three acute cooling stress intensities ( from the temporary culture temperature13 ℃ to 3, 5, 7 ℃ in group Ⅰ,Ⅱ and Ⅲ respectively) and different duration (0,2, 6,12,24 and 48 h) on the serum biochemical parameters of C. striata were analyzed.[Result] The activities of serum ALT, AST, LDH and CK-MB in Ⅰ,Ⅱ groups showed the change trend of first increasing and then decreasing.ALP activity in each group was significantly higher than that before the stress ( P <0.05). TC and TG in the serum in Ⅰ,Ⅱ groups first decreased and then increased up to the level before the stress, and TP, TG, TC contentsin Ⅲ group after12 h were significantly lower than those before the stress ( P <0.05). The glucose(GLU) content in Ⅱ group at 48 h significantly increased ( P <0.05), serum ion in Ⅰ and Ⅱ groups at 48 h had no significant difference with that before the stress( P >0.05). The contentsof K +, Ca2+ in group Ⅲ decreased significantly ( P <0.05), Na + and Cl - contentssignificantly increased ( P <0.05).[Conclusion] The stress intensity and stress time should be reduced as possible, in the process of breeding production and low temperature domestication.
作者
油九菊
李伟业
殷小龙
徐志进
柳敏海
章霞
傅荣兵
YOU Jiu-ju;LI Wei-ye;YIN Xiao-long(Zhoushan Fisheries Research Institute of Zhejiang Province, Zhoushan,Zhejiang 316000)
出处
《安徽农业科学》
CAS
2019年第8期109-113,共5页
Journal of Anhui Agricultural Sciences
基金
海洋经济创新发展区域示范项目(浙海渔计[2015]29号)
舟山市现代渔业发展专项(舟财农[2016]52号)
关键词
条纹锯鮨
急性低温胁迫
血清生化指标
Centropristis striata
Acute low temperature stress
Serumbiochemical parameters
