摘要
目的 构建一种塑化剂邻苯二甲酸二(2-乙基)己酯(di(2-ethylhexyl)phthalate,DEHP)含量测定的荧光探针检测方法。方法 基于芘分子满足激基缔合物的要求,本文选择以芘为荧光探针,设计合成了芘-聚氧乙烯蓖麻油体系(芘-EL体系)。结果 芘荧光探针在该体系中结合DEHP后収生荧光猝灭,且荧光强度的降低与DEHP的浓度之间存在线性兲系。荧光探针检测的最优条件为波长为374 nm, 400μmol/L芘-EL体系,相互作用时间30min。该方法对DEHP测定的线性范围为5～30μmol/L,线性方程为△F=-23.153+24.179C,线性相兲系数r=0.9929,检出限为0.063μmol/L,相对标准偏差为4.43%(n=6)。结论 本方法具有可视化、成本低、高灵敏度、高选择性、操作简便等优点,适用于白酒中DEHP的测定。
Objective To develop a method for the determination of the content of the plasticizer phthalate(di(2-ethylhexyl)phthalate, DEHP) by fluorescence probe detection. Methods Based on the requirement that the pyrene molecule satisfies the radical connective compound, pyrene was selected as the fluorescent probe in this paper,and pyrene-polyoxyethylene castor oil system(pyrene-EL system) was designed and synthesized. Results The fluorescence quenching of pyrene fluorescence probe combined with diethylene phthalate(2-ethyl) hexyl ester(DEHP) occurred in the system, and a linear relationship existed between the reduction of fluorescence intensity and the concentration of DEHP. The optimal condition for fluorescence probe detection was a pyrene-EL system with a wavelength of 374 nm and interaction time of 30 min. The linear range of the method for the determination of DEHP was 5-30 μmol/L, the linear equation was ?F=-23.153+24.179 C(r=0.9929), and the limit of detection was 0.063μmol/L, the relative standard deviation was 4.43%(n=6). Conclusion The method has the advantages of visualization, low cost, high sensitivity, high selectivity and simple operation, which is suitable for the determination of DEHP in liquor.
作者
王燕飞
高雨嫣
陈盛
陈莉
黄丹
王国军
WANG Yan-Fei;GAO Yu-Yan;CHEN Sheng;CHEN Li;HUANG Dan;WANG Guo-Jun(Research Institute of Quality & Technical Supervision and Inspection,Taizhou 318000,China;School of Food Science and Biotechnology,Zhejiang Gongshang University,Hangzhou 310018,China)
出处
《食品安全质量检测学报》
CAS
2019年第6期1607-1613,共7页
Journal of Food Safety and Quality
基金
浙江省质检科研计划项目(20170265)
浙江省台州市科技局项目(1701gy13)
浙江工商大学研究生科研创新基金项目~~
