摘要
目的探讨Rho相关蛋白激酶1(ROCK1)在胶质瘤组织的表达及临床意义。方法采用Western blot检测ROCK1在脑胶质瘤蛋白表达水平,以乘积极限法(Kaplan-Meier)分析ROCK1与脑胶质瘤患者临床特征的关系;以小干扰RNA(siRNA)抑制人神经胶质细胞瘤U251的ROCK1的表达,Transwell迁移实验测定U251细胞迁移侵袭能力,细胞计数试剂盒(CCK-8)检测U251细胞增殖能力。结果免疫印迹结果表明ROCK1在脑胶质瘤组织中高表达。ROCK1的表达与胶质瘤患者性别、年龄和肿瘤大小无明显相关(P>0.05),而ROCK1表达与世界卫生组织(WHO)胶质瘤分级明显相关(P<0.05)。Kaplan-Meier分析显示ROCK1高表达组为13.00±2.04,ROCK1低表达组为25.00±6.98,ROCK1高表达ROCK1的患者预后较差(P<0.01)。Transwell迁移实验结果显示si-ROCK1组跨膜的细胞数量减少45%~50%,表明ROCK1促进肿瘤细胞侵袭;CCK-8法结果显示si-ROCK1组比对照组减少约56个克隆细胞,表明ROCK1促进胶质瘤细胞增殖。结论ROCK1在胶质瘤患者中高表达,与胶质瘤发生、发展和转移明显相关。
Objective To explore the expression and significance of Rho-associated kinase 1 (ROCK1) in glioma. Methods Western blotting was used to detect the protein expression levels of ROCK1 in glioma tissues. Product-Limit (Kaplan-Meier) was used to analyze the relationship between ROCK1 and clinical features of glioma patients. Small interfering RNA (siRNA) interference was applied to inhibit the expression of ROCK1 in glioblastoma cells. The migration of U251 cells was measured by Transwell assay. The proliferation of U251 cells was examined by cell counting kit-8 (CCK-8) assay. Results Western blotting results showed that ROCK1 protein was highly expressed in glioma tissues. The expression of ROCK1 was not significantly correlated with gender, age, and tumor size (P>0.05), but significantly with World health organization (WHO) classification (P<0.05). Kaplan-Meier analysis showed that the median of ROCK1 high expression group was 13.00±2.04, and that of ROCK1 low expression group was 25.00±6.98. Patients with high expression of ROCK1 had a poor prognosis (P<0.01). Transwell assay results showed that there was a 45%-50% reduction in number of transmembrane cells in si-ROCK1 group, indicating that ROCK1 promotes cancer cell invasion. Transwell migration assay results showed the number of transmembrane cells in the si-ROCK1 group was reduced by 45%-50%, indicating that ROCK1 promotes tumor cell invasion. CCK-8 assay results showed that the number of clonal cells in the si-ROCK1 group was reduced by about 56 compared to the control group, indicating that ROCK1 promotes glioma cell proliferation. Conclusion ROCK1 is highly expressed in glioma patients and is associated with the development, progression, and metastasis of glioma.
作者
宋振宇
刘献志
周国胜
马斯奇
罗文正
吴力新
郭孟果
Song Zhenyu;Liu Xianzhi;Zhou Guosheng;Ma Siqi;Luo Wenzheng;Wu Lixin;Guo Mengguo(Department of Neurosurgery, the First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第3期543-545,共3页
Chinese Journal of Experimental Surgery
关键词
Rho相关蛋白激酶1
胶质瘤
侵袭
增殖
Rho-associated protein kinase 1
Glioma
Invasion
Proliferation
