摘要
目的探讨叉头框蛋白K1(FOXK1)在胶质瘤组织的表达及临床意义。方法采用实时定量反转录聚合酶链反应(RT-qPCR)和Western blot检测FOXK1在胶质瘤mRNA和蛋白表达水平;乘积极限法(Kaplan-Meier)分析FOXK1与患者临床病理参数关系;RNA干扰胶质母细胞瘤FOXK1的表达,Transwell迁移实验测定LN18细胞迁移能力,细胞计数试剂盒(CCK-8)细胞计数测定T98G细胞增殖。结果RT-qPCR检测和Western blot结果表明FOXK1在胶质母细胞瘤(GBM)组织中mRNA和蛋白高表达。FOXK1的表达与胶质瘤患者性别无明显相关(P>0.05)、与年龄无明显相关(P>0.05),而FOXK1表达与WHO分级明显相关(P<0.01)。Kaplan-Meier分析显示高表达FOXK1的患者预后较差(P<0.05)。Transwell迁移实验结果表明FOXK1促进癌细胞转移,CCK-8细胞计数结果表明FOXK1促进胶质瘤细胞增殖。结论 FOXK1在胶质瘤患者中高表达,与胶质瘤发生和发展、转移相关。
Objective Explore of the expression and significance of forkhead box protein K1 (FOXK1) in glioma.Methods Real-time quantitative reverse transcriptase-polymerase chain reaction (RT-qPCR) and Western blotting analysis of mRNA and protein expression levels of FOXK1 in glioma tissues;Kaplan-Meier analysis of the relationship between FOXK1 and prognosis parameters;RNA interference on the expression of FOXK1 in glioblastoma cell. Transwell migration assay for the migration of LN18 cells. Cell counting kit-8 (CCK-8) counting for T98G cell proliferation.Results RT-qPCR and Western blotting showed that FOXK1 mRNA and protein is highly expressed in glioma tissues. The expression of FOXK1 was not significantly correlated with gender (P>0.05) and age of glioma patients (P>0.05), while FOXK1 expression was significantly with WHO classification (P<0.01). Kaplan-Meier analysis found that patients with high expression of FOXK1 had a poor prognosis (P<0.05). Transwell migration assay results indicate that FOXO1 promotes cancer cell metastasis. The CCK-8 cell count results indicate that FOXK1 promotes glioma cell proliferation.Conclusion FOXK1 is highly expressed in glioma patients and is associated with glioma development, progression, and metastasis.
作者
宋振宇
周国胜
吴力新
孙逸杰
Song Zhenyu;Zhou Guosheng;Wu Lixin;Sun Yijie(Department of Neurosurgery ,the First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2019年第1期141-143,共3页
Chinese Journal of Experimental Surgery
