摘要
为探讨还原型谷胱甘肽(GSH)参与低温(10℃/6℃)胁迫下一氧化氮(NO)对黄瓜叶绿体抗氧化酶活性、还原力水平及氧化还原状态的影响,本研究以基质培'津研四号'黄瓜幼苗为试材,四叶一心时置光周期14 h/10 h、昼夜温度25℃/20℃光照培养箱内,叶面预处理GSH合成酶抑制剂(BSO,丁硫氨酸亚砜胺)、NADPH合成酶抑制剂(6-AN,6-氨基烟酰胺),8 h后再喷施一氧化氮NO供体(SNP,亚硝基铁氰化钠),第1次喷施药剂后24 h后昼夜温度降至10℃/6℃,低温胁迫24 h后测定各项指标。结果表明:低温胁迫24 h后,与CK相比,水+SNP处理显著提高了黄瓜叶绿体谷胱甘肽还原酶(GR)、过氧化物酶(POD)、抗坏血酸过氧化物酶(APX)、过氧化氢酶(CAT)、脱氢抗坏血酸还原酶(DHAR)、单脱氢抗坏酸还原酶(MDHAR)活性,保持较高的还原型谷胱甘肽/氧化型谷胱甘肽(GSH/GSSG)、还原型抗坏血酸/氧化型抗坏血酸(As A/DHA)、还原型辅酶Ⅱ/氧化型辅酶Ⅱ(NADPH/NADP+)状态,显著降低叶片丙二醛(MDA)含量、叶绿体过氧化氢(H2O2)和超氧阴离子自由基(O2·-)含量;而BSO+SNP、6AN+SNP处理显著降低了SNP的作用效果。本研究结果说明GSH参与了外源NO提高黄瓜叶绿体抗氧化能力的调控。
To investigate the effects of reduced glutathione(GSH) on the antioxidant enzyme activities, reducing power and redox status of chloroplasts in cucumber under low temperature(10℃/6℃) stress.The substrate-cultured ’Jinyan No.4’ cucumber seedlings were used as the test materials.Four-leaf one-hearted photoperiods were set at a photoperiod of 14 h/10 h,day and night temperature of 25℃/20℃ in a light incubator,and leaf surface pretreatment with GSH synthase inhibitors( BSO,butanamine sulfoxide amine),NADPH synthetase inhibitor(6-AN,6-amino nicotinamide),and nitric oxide NO donor(SNP,sodium nitroferrocyanide).The day-night temperature dropped to 10℃/6℃ 24 h after the first spraying, and the indexes were measured after 24 h of cold stress.The results showed that compared with CK, water+SNP treatment significantly increased the activity of glutathione reductase(GR), peroxidase(POD), ascorbate peroxidase(APX), catalase(CAT),and dehydroascorbate reductase(DHAR)and m ono de h y dr oa sc orb ate reductase(MDHAR) in chloroplasts of cucumber, and maintained higher levels of reduced glutathione/oxidized glutathione(GSH/GSSG), reduced ascorbic acid/oxidized ascorbic acid(AsA/DHA) and reduced coenzymeⅡ/oxidized coenzyme Ⅱ(NADPH/NADP+) status,significantly reduced the contents of malondialdehyde(MDA),chloroplast hydrogen peroxide(H202) and superoxide anion(02 ’-).while the treatment of BSO+SNP,6 AN+SNP significantly reduced the effect of SNP.It shows that GSH is involved in the regulation of the antioxidant capacity of cucumber chloroplast by exogenous NO.
作者
索琳格
吴佩
张文博
杨志峰
刘慧英
崔金霞
SUO Linge;WU Pei;ZHANG Wenbo;YANG Zhifeng;LIU Huiying;CUI Jinxia(Xingjiang Production and Construction Corps Key Laboratory of Special Fruits and Vegetables Cultivation Physiology andGermplasm Resources Utilization/College of Agriculture,Shihezi University,Shihezi,Xinjiang 832003 ,China)
出处
《石河子大学学报（自然科学版）》
CAS
北大核心
2018年第5期624-630,共7页
Journal of Shihezi University(Natural Science)
基金
国家自然科学基金项目(31560571)
