摘要
目的建立同时测定心可宁胶囊中羟基红花黄色素A和丹酚酸B含量的方法。方法采用高效液相色谱法(HPLC),色谱柱为Agilent Zorbax Extend C_(18)柱(250 mm×4.6 mm,5μm),柱温30℃,流动相为甲醇-0.5%磷酸(35:65),流速1.0 ml/min,检测波长403 nm(羟基红花黄色素A)、286 nm(丹酚酸B),进样量10μl。结果羟基红花黄色素A在0.1184～0.8880μg的范围内呈良好的线性关系(r=0.9994),丹酚酸B在0.8390～6.2925μg的范围内呈良好的线性关系(r=0.9998);羟基红花黄色素A平均加样回收率为99.17%(n=6),RSD为0.87%,丹酚酸B平均加样回收率为99.41%,RSD为0.87%。结论该方法操作简便、准确、重复性好,可用于心可宁胶囊的质量控制。
Objective To establish a method for the simultancous determination of hydroxysafflor yellow A and salvianolic aicd B in Xinkening Capsules by HPLC.Methods HPLC was performed on an Agilent Zorbax Extend C18(250 mm×4.6 mm,5μm)with the column temperature of 30℃.The moblic phase was methanol-0.5%phosphoric acid(35:65)at a flow rate of 1.0 ml/min,the detection wavelength was 403 nm(for hydroxysafflor yellow A)and 286 nm(for salvianolic aicd B)and the injection volume was 10μl.Results Hydroxysafflor yellow A presented a good linearity in the concentration range of 0.1184-0.8880μg(r=0.9994).Salvianolic aicd B presented a good linearity in the concentration range of 0.8390-6.2925μg(r=0.9998).The average rate recovery of hydroxysafflor yellow A was 99.17%(n=6),with RSD of 0.87%.The average rate recovery aof salvianolic aicd B was 99.41%,with RSD of 0.87%.Conclusion This method is simple,accurate and reproducible,thus can be used for quality control of Xinkening Capsules.
作者
李怀伟
张朋
马昌豪
LI Huai-wei;ZHANG Peng;MA Chang-hao(Heze Institute for Food and Drug Control,Heze 274000,China)
出处
《食品与药品》
CAS
2019年第2期134-137,共4页
Food and Drug
