摘要
目的探讨益气化痰祛瘀中药对慢性间歇性低氧大鼠肝损伤的保护作用。方法将SPF级健康雄性SD大鼠30只,随机分为3组(每组10只),分别为常氧组(常氧并生理盐水灌胃)、慢性间歇性低氧(CIH)组(间歇性低氧并生理盐水灌胃)、CIH+中药组(间歇性低氧并益气化痰祛瘀方药灌胃),8周后取肝脏组织提取蛋白和RNA,行Western免疫印迹和实时定量聚合酶链式反应(PCR)实验检测BAX和BCL-2的蛋白表达和基因表达,采用TUNEL实验检测肝脏细胞凋亡率。结果CIH组中BAX蛋白和基因的表达量较常氧组升高(t=13.490、16.480,P=0.005、0.002)、BCL-2蛋白和基因的表达量较常氧组降低(t=5.142、9.776,P=0.036、0.001);CIH+中药组中BAX蛋白和基因表达量与CIH组比较降低(t=6.088、15.240,P=0.026、0.005)、BCL-2蛋白和基因的表达量与CIH组比较升高(t=5.296、16.380,P=0.034、0.004);CIH组较常氧组肝细胞凋亡率增多(t=7.698,P=0.002),而CIH+中药组较CIH组其肝细胞凋亡率减低(t=4.345,P=0.012)。结论益气化痰祛瘀中药可能抑制肝细胞BAX的表达和增强BCL-2表达,进而减轻慢性间歇性低氧大鼠肝脏细胞的凋亡,对肝脏起到一定的保护作用。
Objective To investigate the protective effects of a Yiqi Huatan Quyu formulation on liver injury in chronic intermittent hypoxia rats. Methods Thirty healthy male Sprague-Dawley rats of SPF grade were randomly divided into 3 groups: Group A(normoxia and normal saline gavage), Group B(chronic intermittent hypoxia and normal saline gavage)and Group C(chronic intermittent hypoxia and Yiqi Huatan Quyu formulation gavage)(n=10 in each group). After 8 weeks of treatment, the liver tissue was extracted for protein and RNA.Western blot was used to test the protein levels of B-cellymphoma-2 associated X protein(BAX)and B-cellymphoma-2(BCL-2), and Realtime PCR was used to test gene expression of BAX and BCL-2.Transferase-mediated dUTP nick end-labeling(TUNEL)was used to assay the apoptotic rate of liver cells. Results The expression of the BAX protein and BAX gene was higher and the expression of the BCL-2 protein and BCL-2 gene was lower in Group B than in Group A(t=13.490 and 16.480, P=0.005 and 0.002;t=5.142 and 9.776, P=0.036 and 0.001, respectively). The levels of the BAX protein and BAX gene were lower and the levels of the BCL-2 protein and BCL-2 gene were higher in Group C than in Group B(t=6.088 and 15.240, P=0.026 and 0.005;t=5.296 and 16.380, P=0.034 and 0.004, respectively). The apoptosis rate of hepatocytes was higher in Group B than in Group A(t=7.698, P=0.002), and was lower in Group C than in Group B(t=4.345, P=0.012). Conclusions The Yiqi Huatan Quyu formulation may alleviate the apoptosis of liver cells in chronic intermittent hypoxia rats by up-regulating BCL-2 and down-regulating BAX levels, thus exerting protective effects on the liver.
作者
冯德怀
陈沁
黄杰凤
陈清石
陈理达
赵建铭
林其昌
Feng Dehuai;Chen Qin;Huang Jiefeng;Chen Qingshi;Chen Lida;Zhao Jianming;Lin Qichang(Department of Respiratory Medicine, the First Affiliated Hospital, Fujian Medical University, Laboratory of Pneumology of Fujian Medical University, Fuzhou 350005, China;Department of Pneumology, People's Hospital Affiliated to Fujian Medical University, Fuzhou 350004, China;Department of Respiratory and Critical Care Medicine, Zhangzhou Affiliated Hospital of Fujian Medical University, Zhangzhou 363000, China)
出处
《中华老年医学杂志》
CAS
CSCD
北大核心
2019年第3期317-321,共5页
Chinese Journal of Geriatrics
基金
福建省自然科学基金(2015J01326)。
