摘要
目的研究IGF-1基因治疗对产后压力性尿失禁大鼠模型的影响,探索对该疾病理想的治疗方法。方法对240只SD雌鼠水囊阴道扩张法建模,从185只成功模型中随机选取148只并分为5组,分别进行IGF-1基因治疗、氨哮素药物治疗、电刺激治疗、空质粒载体注射和未治疗,另选未建模大鼠20只作为空白对照,各组分别于治疗后1、21、42和63 d进行尿动力学检测、血清生化指标(LDH,CK)检测等,并在光镜下观察耻尾肌肌纤维的变化。结果在治疗后21 d时,无论是最大膀胱容量、漏尿点压力还是收缩力/肌重比,IGF-1组、电刺激组治疗效果更佳;而IGF-1组与电刺激组两组之间比较差异无显著性(P>0.05)。结论 IGF-1基因治疗和电刺激治疗对产后压力性尿失禁大鼠模型的效果优于药物治疗等其他组。
Objective To study the effect of IGF-1 gene therapy and electric stimulation therapy on the rat models of postpartum stress urinary incontinence,and explore the ideal treatment for this disease. Methods 240 SD female rats were used to establish the model of postpartum stress urinary incontinence by water sac vaginal dilation. 148 model rats were randomly selected from 185 successful models and divided into 5 groups： IGF-1 gene therapy,clenbuterol treatment,electric stimulation therapy,injection of empty vector plasmid,and untreated groups. Besides,20 non-modeled rats were used as blank control group. Urodynamic test was performed,pelvic floor pubococcygeus muscle / muscle weight ratio was calculated,and serum biochemical indices（ LDH,CK） were detected,and the morphological changes of pubococcygeus muscle fibers were observed by light microscopy at 1,21,42 and 63 days after treatment. Results At 21 days after treatment,the maximum bladder capacity,leak point pressure,the contractile force / muscle weight ratio in the IGF-1 group and electric stimulation treatment group were significantly better（ P〈0. 05）,and the differences between the IGF-1 group and electric stimulation group were not significant（ P〈0. 05）. Conclusions The effect of IGF-1 gene therapy and electric stimulation on the rat models of postpartum stress urinary incontinence is better than that in the drug therapy group and other groups.
出处
《中国实验动物学报》
CAS
CSCD
北大核心
2015年第6期617-621,共5页
Acta Laboratorium Animalis Scientia Sinica
基金
上海市卫生局青年科研项目(编号:20124Y054)
上海市科委西医引导类项目(编号:124119a5501)
上海市申康医院管理集团新兴前沿项目(编号:SHDC12013120)
关键词
IGF-1
基因治疗
产后压力性尿失禁
大鼠模型
IGF-1
Gene therapy
Postpartum stress urinary incontinence
Rat model
Electric stimulation
