摘要
目的对济南市2017年手足口病(hand,foot and mouth disease,HFMD)病原中的柯萨奇病毒A组16型(coxsackievirus A16,CV-A16)分离鉴定,分析VP1基因遗传进化特征。方法整理分析2017年济南市HFMD监测样本,采用简单随机抽样方法选取CV-A16核酸阳性样本经预处理后接种人横纹肌肉瘤(human rhabdomyosarcoma,RD)细胞,对产生明显细胞病变效应的毒株进行鉴定,测定VP1基因序列,应用MEGA5.2软件构建系统进化树,通过Lasergene软件对VP1进行核苷酸及氨基酸同源性分析。结果通过细胞分离获得10株CV-A16阳性病毒株,均属B1b亚型,分别位于3个不同簇,核苷酸及氨基酸序列同源性分别为94.9%~100%和99.3%~100%,与我国CV-A16上海株(KX871333)(同源性99.7%)、河南株(KM260134)(99.0%)、江苏株(KP751580、KR138327)(98.7%)及广东株(MH004016)(98.5%)亲缘关系较近。与国内参考株相比,济南市CV-A16分离株VP1存在N14S、S59R、E145V变异。结论CV-A16病毒株B1b基因亚型为济南市优势毒株,VP1蛋白发生氨基酸变异。
Objective To analyze the VP1 gene characteristics of Coxsackievirus A16 (GV-A16) isolated in (hand,foot and mouth disease,HFMD) patients of Jinan 2017.Methods The samples collected from patients with HFMD in Jinan city in 2017 were analyzed and some of the CV-A16 nucleic acid positive samples were choosen with simple random sampling method and pretreated to inoculate RD cells.The whole VP1 gene sequences of CV-A16 stains which had obvious cytopathic effect in RD cells were amplified and sequenced.MEGA5.2 software was used to constructed phylogenetic tree and Lasergene software was used to analysis the homology consistency of nucleotide and amino acid of VP1 gene.Results All the 10 CV-A16 strains isolated from Jinan were located 3 clusters belonged to Bib genotypes,with a nucleotide similarity of 94.9%-100% and deduced amino acid similarity of 99.3%~100%.The Jinan CV-A16 strains were nearest related with Shanghai strains KX871333 (nucleotide similarity: 99.7%),Henan strains KM260134 (99.0%),Jiangsu strains KP751580,KR138327 (98.7%) and Guangdong strains MH004016 (98.5%).Compared with some reference strains from our country,amino acid mutation were identified at positions 14,59,and 145 in VP1 proteins of Jinan CV-A16 strains.Conclusions CV-A16 strains Bib genotypes were the strains prevailed in Jinan city,and 3 amino acid substitutions in VP1 proteins were happened in CV-A16 strains isolated from Jinan.
作者
赵怀龙
隋竑弢
杨国樑
关恒云
王春荣
刘岚铮
Zhao Huailong;Sui Hongtao;Yang Guoliang;Guan Hengyun;Wang Chunrong;Liu Lanzheng(Jinan Municipal Center for Disease Control and Prevention,Jinan 250021,China;Institute of Pathogen Biology Chinese Academy of Medical Sciences,Beijing 100730,China)
出处
《中华实验和临床病毒学杂志》
CAS
CSCD
2019年第1期44-48,共5页
Chinese Journal of Experimental and Clinical Virology
基金
山东省医药卫生科技发展计划项目(2017WS529)。
