摘要
旨在建立检测饲料中黄曲霉毒素B_1、B_2、G_1、G_2含量的高效液相色谱分析方法。样品用甲醇溶液提取,利用免疫亲和柱净化,以C_(18)反相色谱柱分离,光化学衍生器衍生后应用荧光检测器检测。结果表明:4种黄曲霉毒素在0.5～20 ng/mL范围内呈现良好的线性关系,回收率为71.6%～89.0%。采用该方法检测5种饲料样品中的黄曲霉毒素总量,检出率范围在74.0%～86.7%,检出值的范围为0.5～4.3μg/kg。建立的方法精密度高、重复性好,为监测饲料中黄曲霉毒素含量水平提供了有效的分析方法。
The aim of this study was to establish a high-performance liquid chromatography (HPLC) method for simultaneous determination of aflatoxin B1,B2,G1 and G2 in feed.The sample was extracted with methanol solution,purified by immune-affinity column,separated by C18 reverse phase column,derivatized by photochemical derivatizer,and analyzed by fluorescence detector.The results showed that the four tested aflatoxins showed a good linear relationship in the range of 0.5-20 ng/mL,and the recovery was 71.6%-89.0%.The established HPLC method was used to detect the total amount of aflatoxin in five kinds of feed samples.The detection rate ranged from 74.0% to 86.7%,and the detected value of aflatoxin ranged from 0.5 to 4.3 μg/kg.In conclusion,the established HPLC method has high precision and good repeatability,providing an effective analytical method for monitoring the level of aflatoxin in feed.
作者
赵贞
万鹏
李翠枝
吕志勇
ZHAO Zhen;WAN Peng;LI Cui-zhi;LU Zhi-yong(Inner Mongolia Yili Industrial Group Co.,Ltd.,Hohhot 010010,China)
出处
《畜牧与饲料科学》
2019年第3期27-29,49,共4页
Animal Husbandry and Feed Science
