摘要
目的:对基因芯片表达汇编(GEO)数据库和癌症基因组图谱(TCGA)数据库中的胰腺癌数据进行生物信息学分析,探讨长链非编码RNA(lncRNA)与mRNA在胰腺癌中的差异表达和预后价值。方法:首先对GEO数据库中的胰腺癌数据注释获得mRNA和lncRNA,并对芯片中的mRNA和lncRNA取交集获得差异mRNA和lncRNA。然后对差异mRNA和lncRNA进行基因分析,同时对TCGA数据库中胰腺癌的相关数据进行生存分析。结果:通过基因分析获得与胰腺癌相关可信度高的差异mRNA 1 147个,lncRNA 336个,通过基因本体(GO)和京都基因与基因组百科全书(KEGG)分析,构建lncRNA-mRNA共表达网络、lncRNA-mRNA-pathway网络、信号通路关系网络及蛋白质相互作用网络,结果证明m RNA和lncRNA对胰腺癌的发生发展有着重要影响;生存分析发现有12个lncRNA与胰腺癌的预后相关,分别是ATP1A1-AS1、 CBR3-AS1、 CTD-3080P12.3、 FAM66D、 FAM87A、FLJ38576、LINC00476、LINC00574、LINC01554、PYY2、LINC00857、OVOL1-AS1。结论:lncRNA对胰腺癌的发生发展及预后有显著影响,有望为胰腺癌的靶向治疗和预后评估提供新的治疗靶点和分子标志物。
OBJECTIVE:Using bioinformatics method,the data of pancreatic cancer in Gene Expression Omnibus(GEO)database and The Cancer Genome Atlas(TCGA)database were analyzed to evaluate the differential expression and prognostic value of lncRNA and mRNA in pancreatic cancers.METHODS:Firstly,we annotated the data of pancreatic cancers into GEO database to extract the information on mRNA and lncRNA.By intersecting the mRNA and lncRNA in the chip,we obtained the differentially expressed mRNA and lncRNA.Then,genetic analyses of the differentially expressed mRNA and lncRNA were carried out.At the same time,survival analysis was carried out with the relevant data of pancreatic cancers in the TCGA database.RESULTS:With the gene analyses,we obtained 1 147 differentially expressed mRNAs and 336 differentially expressed IncRNAs,which were reliability related to pancreatic cancer.Through gene ontology analyses and Kyoto encyclopedia of genes and genomes analyses,networks on lncRNA-mRNA co-expression,lncRNA-mRNA-pathways,signal pathways and protein interactions were constructed.The results show that mRNAs and lncRNAs had an important impact on the occurrence and development of pancreatic cancers.Survival analysis revealed that 12 lncRNAs were associated with the prognosis of pancreatic cancer,namely ATP1A1-AS1,CBR3-AS1,CTD-3080P12.3,FAM66D,FAM87A,FLJ38576,LINC00476,LINC00574,LINC01554,PY2,LINC00857,OVOL1-AS1.CONCLUSION:Several LncRNA demonstrated a significant association with the occurrence,development and prognosis of pancreatic cancers.Therefore,they are expected to provide new therapeutic targets and molecular markers for targeted therapy as well as prognosis evaluation of pancreatic cancers.
作者
魏超
张晓
高杰
WEI Chao;ZHANG Xiao;GAO Jie(School of Public Health,Southeast University,Nanjing 210009,Jiangsu,China)
出处
《癌变．畸变．突变》
CAS
2019年第2期119-126,132,共9页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金项目(71373042)
