摘要
目的探讨脓毒症的特征性代谢标志物及代谢网络的动态变化规律。方法采用盲肠结扎穿孔(CLP)方法制作大鼠脓毒症模型,分为A组、B组、C组、D组、E组,每组10只,并分别对应在术前(0h),CLP模型建立后6、12、24、48h采集大鼠股动脉血2mL,离心收集血清;再利用代谢组学分析方法从血清中筛选出具有统计学和生物学意义的代谢物,并以KEGG数据库为基础对筛选出来的代谢物进行代谢通路的分析。结果(1)各组之间负、正离子模式均出现差异离子,与术前(即无脓毒症)对比,脓毒症24h(即D组)差异离子数最多;(2)根据负离子模式确定了75条代谢通路,根据pos离子模式确定了158条代谢通路,代谢通路主要包括碳水化合物、蛋白质、氨基酸、脂类、类固醇类、嘌呤、胆汁酸等各种代谢。结论脓毒症24h代谢组学差异性最明显,有多条碳水化合物、蛋白质、氨基酸等代谢通路参与。
Objective To explore the characteristic metabolic markers of sepsis and the dynamic change rule of metabolic network.Methods The rat sepsis models were prepared by using the cecal ligation and puncture(CLP),which were divided into the group A,B,C,D and E,10 rats in each group,2mL femoral arterial blood was collected before operation(0 h),at 6,12,24,48 h after the CLP model construction respectively,and centrifuged for collecting serum;then the metabolomics analysis method was utilized to screen out metabolites with statistical and biological significance from the serum and its metabolic pathway analysis on the screened metabolites was performed based on the KEGG database.Results(1)The negative and positive ion modes among the groups all showed the different ions,and the number of different ions at 24 h in sepsis(group D)was maximal compared with preoperation(non-sepsis).(2)According to the negative ion mode,75 metabolic pathways were determined,and 158 metabolic pathways were determined according to the positive ion mode.The metabolic pathways mainly included various metabolisms such as carbohydrates,protein,amino acids,lipids,cholesterol,purine and bile acid.Conclusion The difference of sepsis 24 h metabolomics is most distinct with many metabolic pathways participation such as carbohydrates,proteins,amino acid.
作者
周凤高
徐冕
颜悦新
刘桠名
许成
蒋国云
赵琨
武彧
刘荣
ZHOU Fenggao;XU Mian;YAN Yuexin;LIU Yaming;XU Cheng;JIANG Guoyun;ZHAO Kun;WU Yu;LIU Rong(Emergency Intensive Care Unit,First Affiliated Hospital of Kunming Medical University,Kunming,Yunnan 650032,China;Surgical Intensive Care Unit,Fuwai Cardiovascular Hospital of Yunnan Province,Kunming,Yunnan 650032,China;Intensive Care Unit,Affiliated Shenzhen Baoan Hospital of Southern Medical University,Shenzhen,Guangdong 518100,China)
出处
《重庆医学》
CAS
2019年第5期765-769,774,共6页
Chongqing medicine
基金
云南省科技厅-昆明医科大学应用基础研究联合专项(2015FB022)
关键词
脓毒症
盲肠结扎穿孔
代谢组学
液相色谱-质谱
sepsis
cecal ligation and puncture
metabolomics
liquid chromatography-mass spectrometry
